Mouse anti-Human KIR2DL4 Monoclonal Antibody | anti-KIR2DL4 antibody

KIR2DL4 (p58 KIR, CD158, KIR-103AS, Killer Cell Immunoglobulin (Ig)-like Receptor) (PE)

Cat. Num. AAA602227

• Gene Names: KIR2DL4; G9P; CD158D; KIR103; KIR103AS
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified; Purified.
• Synonyms: KIR2DL4; Monoclonal Antibody; KIR2DL4 (p58 KIR; CD158; KIR-103AS; Killer Cell Immunoglobulin (Ig)-like Receptor) (PE); Anti -KIR2DL4 (p58 KIR; anti-KIR2DL4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2A
• Clone Number: 8B31
• Specificity: Recognizes human KIR2DL4.
• Purity/Purification: Purified; Purified.
• Form/Format: Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with R-PHYCOERYTHRIN (PE).

• Applicable Applications for anti-KIR2DL4 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry.; Dilution: Flow Cytometry: 1:10ul labels 1x10e5 cells.
• Immunogen: Human KIR2DL4-transfected BaF/3 cells.
• Preparation and Storage: May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4 degree C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

Related Product Information for anti-KIR2DL4 antibody

KIR2DL4 (2DL4, p49, CD158d, KIR103) is a type I transmembrane protein of the killer cell Ig-like receptor (KIR) family expressed on NK and subsets of gamma delta T and memory/effector abT cells. KIR2DL4 is a unique KIR;1-3 alleles are not clonally restricted but are expressed codominantly4 in all activated NK cells and constitutively on CD56hi NK cells. KIR members with two Ig-like domains (2D) usually express domains D1 and D2, but KIR2DL4 expresses D0 and D2. Other long-tailed (L) KIR have two cytoplasmic inhibitory signaling domains (ITIM), but KIR2DL4 has one ITIM and also exhibits characteristics of activating KIR.2 An arginine within the transmembrane sequence of KIR2DL4 interacts with the signaling molecule Fc RI-g, while in activating KIR, a transmembrane lysine interacts with DAP12.1, 5 The KIR2DL4 gene is highly polymorphic. Seven splice variants missing one or more exons have been identified, but it is not clear whether these are expressed. Several of the nine alleles identified encode a frameshift creating a prematurely truncated protein; it is estimated that up to 25% of humans do not express KIR2DL4 capable of reaching the cell surface.1, 7, 10 Human KIR2DL4 is 65%-83% amino acid identical to other primates. KIR receptors have no structural orthologs in non-primates, although mouse Ly49 proteins are functional orthologs. Cross-linking of KIR2DL4 induces NK cells to produce IFN- ;6, 7 stimulation with IL-2 upregulates cell surface expression on CD56dim cells and allows cytotoxicity.7 Although a role in immune privilege of the fetus has been suggested due to reported recognition of fetal trophoblast HLA-G by KIR2DL4 in the maternal decidua,11 subsequent data have not supported this recognition.1, 9

Product Categories/Family for anti-KIR2DL4 antibody

Antibodies; Abs to CD Markers

NCBI and Uniprot Product Information

• NCBI GI #: 56968179
• NCBI GeneID: 3805
• UniProt Accession #: Q5IH87; O00547; O43803; Q32WD9; Q32WE0; Q32WE1; Q32WE2; Q5IH82; Q5IHW5; Q5IHW6
• Molecular Weight: 29,169 Da
• NCBI Official Full Name: KIR2DL4
• NCBI Official Synonym Full Names: killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 4
• NCBI Official Symbol: KIR2DL4
• NCBI Official Synonym Symbols: G9P; CD158D; KIR103; KIR103AS
• NCBI Protein Information: killer cell immunoglobulin-like receptor 2DL4; KIR-103AS; NK cell receptor; OTTHUMP00000068610; OTTHUMP00000068611; OTTHUMP00000068612; OTTHUMP00000068614; OTTHUMP00000068615; OTTHUMP00000068616; killer Ig receptor; CD158 antigen-like family member D; MHC class I NK cell receptor KIR103AS; killer cell inhibitory receptor 103AS; natural killer cell inhibitory receptor
• UniProt Protein Name: KIR2DL4
• Protein Family: Killer cell immunoglobulin-like receptor
• UniProt Gene Name: KIR2DL4
• UniProt Entry Name: Q5IH87_HUMAN

NCBI Description

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. This gene is one of the "framework" loci that is present on all haplotypes. Alternative splicing results in multiple transcript variants. [provided by RefSeq]