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Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded rat breast cancer stained with p130 Ab using peroxidase-conjugate and AEC chromogen. Note cytoplasmic and membrane staining of tumor cells.)
Mouse anti-Human, Rat p130Cas Monoclonal Antibody | anti-p130CAS antibody
p130Cas (Cas for Crk-associated substrate, BCAR1, K-associated Substrate, Breast Cancer anti-estrogen Resistance 1 Protein) (BSA & Azide Free)
Gene Names
p130CAS; CG1212; CT1293; Dcas; DCas; DmelCG1212
Reactivity
Human, Rat
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
p130Cas; Monoclonal Antibody; p130Cas (Cas for Crk-associated substrate; BCAR1; K-associated Substrate; Breast Cancer anti-estrogen Resistance 1 Protein) (BSA & Azide Free); Anti -p130Cas (Cas for Crk-associated substrate; anti-p130CAS antibody
Host
Mouse
Reactivity
Human, Rat
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3F309
Specificity
Recognizes human p130Cas. Species Crossreactivity: rat.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See P1001-97E.
Applicable Applications for anti-p130CAS antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Immunoprecipitation, Western Blot and Immunhistochemistry.
Dilution: Immunoprecipitation (Denatured verified): 2ug/mg protein lysate. (Use Protein G).
Western Blot:1-2ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilution determined by the researcher.
Dilution: Immunoprecipitation (Denatured verified): 2ug/mg protein lysate. (Use Protein G).
Western Blot:1-2ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilution determined by the researcher.
Immunogen
Recombinant human p130cas protein. MW of Antigen: 130kD. Cellular Localization: Cytoplasmic.
Cellular Localization
Cytoplasmic.
Positive Control
MCF-7 cells, HUVEC cells. Tonsil or breast carcinoma.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20 degree C. Aliquots are stable for at least 12 months at-20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded rat breast cancer stained with p130 Ab using peroxidase-conjugate and AEC chromogen. Note cytoplasmic and membrane staining of tumor cells.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded rat breast cancer stained with p130 Ab using peroxidase-conjugate and AEC chromogen. Note cytoplasmic and membrane staining of tumor cells.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human breast cancer stained with p130 Ab using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human breast cancer stained with p130 Ab using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.)
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for anti-p130CAS antibody
The p130Cas (Cas for Crk-associated substrate) is a common cellular target of phosphorylation signal via v-Crk and v-Src oncoproteins. p130Cas has a unique structure that contains a Src homology (SH)-3 domain followed by multiple YXXP motifs and a proline-rich region. p130Cas is implicated in a variety of biological processes including cell adhesion, cell migration, growth factor stimulation, cytokine receptor engagement and bacterial infection. Physiological functions of p130Cas include cardiovascular development, actin filament assembly and Src-induced cell transformation.
Product Categories/Family for anti-p130CAS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
p130CAS, isoform G
NCBI Official Symbol
p130CAS
NCBI Official Synonym Symbols
CG1212; CT1293; Dcas; DCas; DmelCG1212
NCBI Protein Information
CG1212 gene product from transcript CG1212-RD; CG1212-PB; CG1212-PD; CG1212-PE; CG1212-PF; CG1212-PG; p130CAS; p130CAS-PB; p130CAS-PD; p130CAS-PE; p130CAS-PF; p130CAS-PG
Research Articles on p130CAS
Similar Products
Product Notes
The p130CAS (Catalog #AAA602206) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The p130Cas (Cas for Crk-associated substrate, BCAR1, K-associated Substrate, Breast Cancer anti-estrogen Resistance 1 Protein) (BSA & Azide Free) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's p130Cas can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). Suitable for use in Immunoprecipitation, Western Blot and Immunhistochemistry. Dilution: Immunoprecipitation (Denatured verified): 2ug/mg protein lysate. (Use Protein G). Western Blot:1-2ug/ml for 2hrs at RT Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilution determined by the researcher. Researchers should empirically determine the suitability of the p130CAS for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p130Cas, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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