SDS-Page
(Detection of p130Cas with MBS622804. Lysates from human HeLa, mouse NIH3T3, and rat L6 cells were resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml sheep anti-p130Cas.)
Sheep p130Cas Polyclonal Antibody | anti-p130CAS antibody
p130Cas (Cas for Crk-associated substrate, BCAR1, K-associated Substrate, Breast Cancer anti-estrogen Resistance 1 Protein)
Gene Names
p130CAS; CG1212; CT1293; Dcas; DCas; DmelCG1212
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
p130Cas; Polyclonal Antibody; p130Cas (Cas for Crk-associated substrate; BCAR1; K-associated Substrate; Breast Cancer anti-estrogen Resistance 1 Protein); Anti -p130Cas (Cas for Crk-associated substrate; anti-p130CAS antibody
Host
Sheep
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes endogenous human p130Cas at 105-130kD. Species Crossreactivity: mouse, rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile PBS, sterile 40-50% glycerol, 0.02% sodium azide.
Applicable Applications for anti-p130CAS antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1ug/ml
Dilution: Western Blot: 1ug/ml
Immunogen
E. coli-derived recombinant human p130Cas (aa66-412).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS, sterile 40-50% glycerol, 0.02% sodium azide, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(Detection of p130Cas with MBS622804. Lysates from human HeLa, mouse NIH3T3, and rat L6 cells were resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml sheep anti-p130Cas.)
SDS-Page
(Detection of p130Cas with MBS622804. Lysates from human HeLa, mouse NIH3T3, and rat L6 cells were resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml sheep anti-p130Cas.)
Related Product Information for anti-p130CAS antibody
p130Cas (protein 130kD Crk-associated substrate; also BCAR1) is a cytoplasmic member of the CAS family of proteins. Although its predicted MW is 93kD, it runs anomalously at 105-130kD in SDS-PAGE. It is found in a wide variety of cell types. p130Cas is involved in cell spreading and migration (but not adhesion), and its cleavage into a 31kD fragment promotes anoikis and cell death. Human p130Cas is 870aa in length. It contains one SH3 domain (aa3-65), a Pro-rich region (aa74-87), at least 10 phosphotyrosine (aa128-410) and four phosphoserine (aa134-639) sites, an SH3 binding motif (aa635-643) and a HLH dimerization segment that interacts with CASL.
Product Categories/Family for anti-p130CAS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
85,622 Da
NCBI Official Full Name
p130CAS, isoform G
NCBI Official Symbol
p130CAS
NCBI Official Synonym Symbols
CG1212; CT1293; Dcas; DCas; DmelCG1212
NCBI Protein Information
CG1212 gene product from transcript CG1212-RA; p130CAS, isoform A; CG1212-PA; p130CAS-PA; p130CAS, isoform D; CG1212-PD; p130CAS-PD; p130CAS, isoform G; CG1212-PG; p130CAS-PG; p130CAS, isoform B; CG1212-PB; p130CAS-PB; p130CAS, isoform F; CG1212-PF; p130CAS-PF; p130CAS, isoform E; CG1212-PE; p130CAS-PE
UniProt Protein Name
Crk-associated substrate
UniProt Gene Name
p130CAS
UniProt Entry Name
Q7KVE5_DROME
Uniprot Description
Sequence similarities: Contains 1 SH3 domain. RuleBase RU003338
Research Articles on p130CAS
Similar Products
Product Notes
The p130CAS p130cas (Catalog #AAA622804) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The p130Cas (Cas for Crk-associated substrate, BCAR1, K-associated Substrate, Breast Cancer anti-estrogen Resistance 1 Protein) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's p130Cas can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1ug/ml. Researchers should empirically determine the suitability of the p130CAS p130cas for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p130Cas, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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