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Rat anti-Human, Mouse Wnt9b Monoclonal Antibody | anti-WNT9B antibody

Wnt9b (Wnt-14b, Wnt-15, Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)

Gene Names
WNT9B; WNT15; WNT14B
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Synonyms
Wnt9b; Monoclonal Antibody; Wnt9b (Wnt-14b; Wnt-15; Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member); Anti -Wnt9b (Wnt-14b; anti-WNT9B antibody
Ordering
For Research Use Only!
Host
Rat
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
7H230
Specificity
Recognizes mouse Wnt9b. Does not cross react with recombinant human Wnt2, -6, -7a, -7b, -9a, recombinant mouse Wnt1, -2b, -4, -5a, -5b, -8a, -8b, -10a, -10b, -11 or -16.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Applicable Applications for anti-WNT9B antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot and ELISA.
Dilution: Western Blot: 1-5ug/ml. Using a colormetric detection system, the detection limit is ~25ng/lane under non-reducing and reducing conditions. Used at 5ug/ml to detect Wnt9b in cell extracts.
Direct ELISA: 0.5-1ug/ml. The detection limit is ~100ng/well.
Immunogen
Recombinant corresponding to aa46-90 and 228-298 connected by a gly-ser linker from mouse Wnt9b expressed in E. coli (Accession #NP_035849).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Related Product Information for anti-WNT9B antibody
Mouse Wnt9b is a presumably secreted, glycosylated member of the Wnt family of developmental proteins. The 359aa mouse Wnt9b precursor contains a 336aa mature region with 24 conserved cysteines and one potential N-linked glycosylation site. Mature mouse Wnt9b shows 93% and 98% aa identity with mature human and rat Wnt9b, respectively, and 63% aa identity with Wnt9a. Wnt9b is expressed in late embryos and in adult kidney, with lesser amounts in brain and liver.
Product Categories/Family for anti-WNT9B antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
39,001 Da
NCBI Official Full Name
WNT9B
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 9B
NCBI Official Symbol
WNT9B
NCBI Official Synonym Symbols
WNT15; WNT14B
NCBI Protein Information
protein Wnt-9b; protein Wnt-14b; wingless-type MMTV integration site family, member 15
UniProt Protein Name
Protein Wnt-9b
Protein Family
UniProt Gene Name
WNT9B
UniProt Synonym Gene Names
WNT14B; WNT15
UniProt Entry Name
WNT9B_HUMAN

NCBI Description

The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Study of its expression in the teratocarcinoma cell line NT2 suggests that it may be implicated in the early process of neuronal differentiation of NT2 cells induced by retinoic acid. This gene is clustered with WNT3, another family member, in the chromosome 17q21 region. [provided by RefSeq]

Uniprot Description

WNT9B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: frizzled binding

Biological Process: positive regulation of catalytic activity; regulation of protein amino acid phosphorylation; cell fate commitment; response to retinoic acid; Wnt receptor signaling pathway; regulation of asymmetric cell division; Wnt receptor signaling pathway, planar cell polarity pathway; in utero embryonic development; multicellular organismal development; embryonic cranial skeleton morphogenesis; palate development; Wnt receptor signaling pathway through beta-catenin; cellular response to starvation; neuron differentiation; cell-cell signaling; ureteric bud branching; male genitalia development; regulation of tube size

Research Articles on WNT9B

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Product Notes

The WNT9B wnt9b (Catalog #AAA601754) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Wnt9b (Wnt-14b, Wnt-15, Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Wnt9b can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot and ELISA. Dilution: Western Blot: 1-5ug/ml. Using a colormetric detection system, the detection limit is ~25ng/lane under non-reducing and reducing conditions. Used at 5ug/ml to detect Wnt9b in cell extracts. Direct ELISA: 0.5-1ug/ml. The detection limit is ~100ng/well. Researchers should empirically determine the suitability of the WNT9B wnt9b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Wnt9b, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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