Western Blot (WB)
(Western Blot analysis using MBS601523.)
Mouse anti-Human MGMT Monoclonal Antibody | anti-Mgmt antibody
MGMT (O6-Methylguanine DNA Methyltransferase)
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
MGMT; Monoclonal Antibody; MGMT (O6-Methylguanine DNA Methyltransferase); Anti -MGMT (O6-Methylguanine DNA Methyltransferase); anti-Mgmt antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
0.N.411
Specificity
Recognizes human MGMT (O6-methylguanine-DNA methyltransferase). Species Crossreactivity: Weakly crossreacts with mouse, rat and hamster.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Applicable Applications for anti-Mgmt antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry.
Dilution: Western Blot: 0.5-1ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1:10-1:20 for 60 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilution to be determined by the researcher.
Dilution: Western Blot: 0.5-1ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1:10-1:20 for 60 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilution to be determined by the researcher.
Immunogen
Recombinant human MGMT protein. Cellular Localization: Nuclear and cytoplasmic. MW of Antigen: 25kD. Epitope: aa8-221
Positive Control
Raji cells; Colon carcinoma
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot analysis using MBS601523.)
Western Blot (WB)
(Western Blot analysis using MBS601523.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human colon carcinoma stained with MBS601523 using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human colon carcinoma stained with MBS601523 using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.)
Related Product Information for anti-Mgmt antibody
Cancer chemotherapeutic alkylating agents (e.g. BCNU) act by inducing formation of lethal cross-links at the O-alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6-position of guanine in DNA (prior to cross-link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA-damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely be drug-resistant.
Product Categories/Family for anti-Mgmt antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
22,244 Da
NCBI Official Full Name
Methylated-DNA--protein-cysteine methyltransferase
NCBI Official Synonym Full Names
O-6-methylguanine-DNA methyltransferase
NCBI Official Symbol
Mgmt
NCBI Protein Information
methylated-DNA--protein-cysteine methyltransferase; O6-alkylguanine-DNA alkyltransferase; O6-methylguanine-DNA methyltranferase; O-6-methylguanine-DNA-alkyltransferase; 0-6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase
UniProt Protein Name
Methylated-DNA--protein-cysteine methyltransferase
Protein Family
UniProt Gene Name
Mgmt
UniProt Synonym Gene Names
MGMT
UniProt Entry Name
MGMT_RAT
NCBI Description
enzyme involved in DNA repair of O(6)-alkylguanine which is the major mutagenic and carcinogenic lesion in DNA [RGD, Feb 2006]
Uniprot Description
MGMT: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Belongs to the MGMT family.
Protein type: EC 2.1.1.63; Apoptosis; DNA repair, damage; Methyltransferase; Nuclear receptor co-regulator
Cellular Component: nucleoplasm; membrane; nucleus
Molecular Function: methyltransferase activity; methylated-DNA-[protein]-cysteine S-methyltransferase activity; damaged DNA binding; calcium ion binding
Biological Process: response to drug; methylation; response to ethanol; positive regulation of DNA repair; DNA dealkylation; response to toxin; response to folic acid; regulation of caspase activity; DNA repair; response to organic cyclic substance; response to DNA damage stimulus
Research Articles on Mgmt
Similar Products
Product Notes
The Mgmt mgmt (Catalog #AAA601523) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MGMT (O6-Methylguanine DNA Methyltransferase) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MGMT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Dilution: Western Blot: 0.5-1ug/ml for 2hrs at RT Immunohistochemistry (Formalin/paraffin): 1:10-1:20 for 60 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Optimal dilution to be determined by the researcher. Researchers should empirically determine the suitability of the Mgmt mgmt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MGMT, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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