Sheep anti-Human PRELP Polyclonal Antibody | anti-PRELP antibody

PRELP (Prolargin, Proline-arginine-rich End Leucine-rich Repeat Protein, SLRR2A)

Cat. Num. AAA6013111

• Gene Names: PRELP; MST161; SLRR2A; MSTP161
• Reactivity: Human
• Applications: Western Blot, Neutralization Assay
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PRELP; Polyclonal Antibody; PRELP (Prolargin; Proline-arginine-rich End Leucine-rich Repeat Protein; SLRR2A); Anti -PRELP (Prolargin; anti-PRELP antibody

• Host: Sheep
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRELP.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.

• Applicable Applications for anti-PRELP antibody: Western Blot (WB), Neutralization
• Application Notes: Suitable for use in Western Blot and Neutralization.; Dilution: Western Blot: 1ug/ml
• Immunogen: Recombinant corresponding to aa21-382 from human PRELP expressed in CHO cell line.
• Endotoxin Level: ~0.1EU/1ug (LAL)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(PRELP Inhibition of TRANCE-dependent Osteoclast-Like Cell Formation and Neutralization by Human PRELP Antibody. Recombinant Human PRELP inhibits Recombinant Mouse TRANCE induced osteoclast-like cell formation in the RAW 264.7 mouse monocyte/macrophage cell line in a dose-dependent manner (orange line), as measured by the TRAP (tartrate-resistant acid phosphatase) solution assay. Inhibition of Recombinant Mouse TRANCE (5ng/ml) activity elicited by Recombinant Human PRELP (5ug/ml) is neutralized (green line) by increasing concentrations of Sheep Human PRELP Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.2-1.2ug/ml in the presence of Recombinant Mouse M-CSF(20ng/ml).)

Western Blot (WB)

(Detection of Human PRELP by Western Blot. Western blot shows lysates of human liver tissue and human pancreas tissue. PVDF membrane was probed with 1ug/ml of Sheep Human PRELP Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Sheep IgG Secondary Antibody. A specific band was detected for PRELP at approximately 65kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer.)

Related Product Information for anti-PRELP antibody

PRELP is a 55-62kD secreted glycoprotein that belongs to the small leucine rich proteoglycan superfamily of extracellular matrix molecules. Within this family, it is considered a class II member, implying that it is unlikely to form dimeric structures. PRELP is synthesized as a 382aa precursor that contains a 20aa signal sequence plus a 362aa mature region. Like other SLRPs, PRELP contains an N-terminal extension aa72-107 coupled to multiple Leucine-rich aa95-382. Unlike other SLRPs, PRELP does not contain any proteoglycan chains, and its N-terminal extension is highly basic in charge. The N-terminus reportedly binds to negatively charged heparin/heparin sulfate, chondroitin sulfate, and Gram bacterial cell walls, while the LRR region participates in protein_protein interactions. Although PRELP is known to be synthesized by only a few cell types, including osteoblasts, skeletal muscle and chondrocytes, its expression is likely to be more widespread, given its presence in the basement membrane (BM) of Bowman's capsule, epididymal epithelium and the stratified squamous epithelium of the skin. The dual binding profile of PRELP is key to its function. In cartilage, PRELP likely links chondrocyte cell membrane heparin sulfate (HS) chains to endogenous type II collagen. Within the context of the BM, PRELP likely plays an anchoring role. The BM is composed of type IV collagen and laminin, linked together by nidogen. BM Perlecan reinforces this linkage by binding to all three components. PRELP, on the edge of the BM, can bind to free perlecan HS chains (via its N-terminus), and to underlying type I collagen (via its LRRs), thus forming an anchor for the BM. Notably, the N-terminus appears to do more than simply provide part of a linkage mechanism. In bone, osteoblast secreted PRELP is hypothesized to undergo proteolysis by enzymes such as LysC and glutamyl endopeptidase. This will generate aa40-75 N-terminal fragments that can bind to chondroitin sulfate adducts that exist on the surface of prefusion osteoclast precursors. Following binding, PRELP is internalized, complexed to annexin_II, and translocated to the nucleus, where it interacts with NF-Bp65 to block osteoclast maturation. In tissue, PRELP may also undergo proteolytic processing during inflammation to release an N--terminal fragment containing aa21-42 of the precursor. This sequence has been shown to possess potent antimicrobial activity by creating pores in bacterial cell walls. Mature human PRELP shares 91% aa identity with mouse PRELP.

Product Categories/Family for anti-PRELP antibody

Antibodies; Abs to Leucine-rich Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 41349454
• NCBI GeneID: 5549
• NCBI Accession #: NP_958505.1
• NCBI GenBank Nucleotide #: NM_201348.1
• UniProt Accession #: P51888; Q6FG38
• Molecular Weight: 43,810 Da
• NCBI Official Full Name: prolargin
• NCBI Official Synonym Full Names: proline/arginine-rich end leucine-rich repeat protein
• NCBI Official Symbol: PRELP
• NCBI Official Synonym Symbols: MST161; SLRR2A; MSTP161
• NCBI Protein Information: prolargin; prolargin proteoglycan; proline-arginine-rich end leucine-rich repeat protein; 55 kDa leucine-rich repeat protein of articular cartilage
• UniProt Protein Name: Prolargin
• Protein Family: Prolargin
• UniProt Gene Name: PRELP
• UniProt Synonym Gene Names: SLRR2A
• UniProt Entry Name: PRELP_HUMAN

NCBI Description

The protein encoded by this gene is a leucine-rich repeat protein present in connective tissue extracellular matrix. This protein functions as a molecule anchoring basement membranes to the underlying connective tissue. This protein has been shown to bind type I collagen to basement membranes and type II collagen to cartilage. It also binds the basement membrane heparan sulfate proteoglycan perlecan. This protein is suggested to be involved in the pathogenesis of Hutchinson-Gilford progeria (HGP), which is reported to lack the binding of collagen in basement membranes and cartilage. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

PRELP: May anchor basement membranes to the underlying connective tissue. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular matrix; lysosomal lumen; proteinaceous extracellular matrix; Golgi lumen; extracellular region; vesicle

Molecular Function: heparin binding; extracellular matrix structural constituent

Biological Process: keratan sulfate metabolic process; glycosaminoglycan metabolic process; keratan sulfate biosynthetic process; carbohydrate metabolic process; cell aging; pathogenesis; keratan sulfate catabolic process; skeletal development