Rabbit anti-Human ENC1 Polyclonal Antibody | anti-ENC1 antibody

ENC1, CT (ENC1, KLHL37, NRPB, PIG10, Ectoderm-neural cortex protein 1, Kelch-like protein 37, Nuclear matrix protein NRP/B, p53-induced gene 10 protein)

Cat. Num. AAA6012226

• Gene Names: ENC1; NRPB; CCL28; ENC-1; PIG10; KLHL35; KLHL37; TP53I10
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: ENC1; Polyclonal Antibody; CT (ENC1; KLHL37; NRPB; PIG10; Ectoderm-neural cortex protein 1; Kelch-like protein 37; Nuclear matrix protein NRP/B; p53-induced gene 10 protein); Anti -ENC1; anti-ENC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-ENC1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: ENC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-423 amino acids from the C-terminal region of human ENC1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ENC1 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ENC1 antibody detected the ENC1 protein (arrow).)

Related Product Information for anti-ENC1 antibody

DNA damage and/or hyperproliferative signals activate wildtype p53 tumor suppressor protein (TP53; MIM 191170), inducing cell cycle arrest or apoptosis. Mutations that inactivate p53 occur in 50% of all tumors. Polyak et al. (1997) [PubMed 9305847] used serial analysis of gene expression (SAGE) to evaluate cellular mRNA levels in a colorectal cancer cell line transfected with p53. Of 7,202 transcripts identified, only 14 were expressed at levels more than 10-fold higher in p53-expressing cells than in control cells. Polyak et al. (1997) [PubMed 9305847] termed these genes 'p53-induced genes,' or PIGs, several of which were predicted to encode redox-controlling proteins. They noted that reactive oxygen species (ROS) are potent inducers of apoptosis. Flow cytometric analysis showed that p53 expression induces ROS production, which increases as apoptosis progresses under some conditions. The authors stated that the PIG10 gene, also called ENC1, encodes an actin-binding protein.

Product Categories/Family for anti-ENC1 antibody

Antibodies; Abs to Kelch-like Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 375268727
• NCBI GeneID: 8507
• NCBI Accession #: NP_001243505.1
• NCBI GenBank Nucleotide #: NM_001256576.1
• UniProt Accession #: O14682; O75464; Q9UPG9; B4DHJ1; E9PFU0
• Molecular Weight: 66,130 Da
• NCBI Official Full Name: ectoderm-neural cortex protein 1 isoform 2
• NCBI Official Synonym Full Names: ectodermal-neural cortex 1 (with BTB domain)
• NCBI Official Symbol: ENC1
• NCBI Official Synonym Symbols: NRPB; CCL28; ENC-1; PIG10; KLHL35; KLHL37; TP53I10
• NCBI Protein Information: ectoderm-neural cortex protein 1; kelch-like 35; kelch-like protein 37; kelch-like family member 37; p53-induced gene 10 protein; nuclear matrix protein NRP/B; tumor protein p53 inducible protein 10; ectodermal-neural cortex 1 (with BTB-like domain); nuclear restricted protein, BTB domain-like (brain)
• UniProt Protein Name: Ectoderm-neural cortex protein 1
• Protein Family: Ectoderm-neural cortex protein
• UniProt Gene Name: ENC1
• UniProt Synonym Gene Names: KLHL37; NRPB; PIG10; ENC-1
• UniProt Entry Name: ENC1_HUMAN

NCBI Description

This gene encodes a member of the kelch-related family of actin-binding proteins. The encoded protein plays a role in the oxidative stress response as a regulator of the transcription factor Nrf2, and expression of this gene may play a role in malignant transformation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

ENC1: Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. May be down-regulated in neuroblastoma tumors. Substrate- specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

Protein type: Actin-binding

Chromosomal Location of Human Ortholog: 5q13

Cellular Component: nucleoplasm; nuclear matrix; cytoskeleton; cell soma; nuclear chromatin; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; actin binding

Biological Process: nervous system development; negative regulation of translation; multicellular organismal development; proteasomal ubiquitin-independent protein catabolic process; protein ubiquitination