Goat anti-Human Vesicle Protein Sorting 35 Polyclonal Antibody | anti-Vps35 antibody

Vesicle Protein Sorting 35, CT (Vacuolar Protein Sorting-associated Protein 35, VPS35, hVPS35, Maternal-embryonic 3, MEM3, TCCCTA00141)

Cat. Num. AAA6012158

• Gene Names: Vps35; Mem3; AI647796
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Vesicle Protein Sorting 35; Polyclonal Antibody; CT (Vacuolar Protein Sorting-associated Protein 35; VPS35; hVPS35; Maternal-embryonic 3; MEM3; TCCCTA00141); Anti -Vesicle Protein Sorting 35; anti-Vps35 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human VPS35. Species sequence homology: bovine, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-Vps35 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:32,000; Western Blot: 0.01-1ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 3.75ug/ml
• Immunogen: Synthetic peptide C-SPESEGPIYEGLIL from the C-terminus of human VPS35.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Breast, Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE))

Chemiluminescence

(Staining (0.05ug/ml) of human brain cerebellum lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.)

Related Product Information for anti-Vps35 antibody

VPS35, otherwise known as MEM3, is a ubiquitously expressed member of the vacuolar protein sorting (VPS) family, and vital component of the multimeric retromer complex, involved in the retrieval of lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans Golgi network.

Product Categories/Family for anti-Vps35 antibody

Antibodies; Abs to Transport Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 13928670
• NCBI GeneID: 65114
• NCBI Accession #: NP_075373.1
• NCBI GenBank Nucleotide #: NM_022997.4
• UniProt Accession #: Q9EQH3; Q61123
• Molecular Weight: 91,713 Da
• NCBI Official Full Name: vacuolar protein sorting-associated protein 35
• NCBI Official Synonym Full Names: vacuolar protein sorting 35
• NCBI Official Symbol: Vps35
• NCBI Official Synonym Symbols: Mem3; AI647796
• NCBI Protein Information: vacuolar protein sorting-associated protein 35; maternal-embryonic 3; vesicle protein sorting 35; maternal embryonic message 3
• UniProt Protein Name: Vacuolar protein sorting-associated protein 35
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: Vps35
• UniProt Synonym Gene Names: Mem3
• UniProt Entry Name: VPS35_MOUSE

Uniprot Description

VPS35: Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Defects in VPS35 are the cause of Parkinson disease type 17 (PARK17). PARK17 is an autosomal dominant, adult- onset form of Parkinson disease. Parkinson disease is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. Belongs to the VPS35 family.

Protein type: Vesicle

Cellular Component: membrane; lysosomal membrane; retromer complex; late endosome; cytoplasm; integral to membrane; vacuole; cytosol; endosome

Molecular Function: protein binding; protein transporter activity

Biological Process: intracellular protein transport; protein transport; transport; vacuolar protein processing; transcytosis; retrograde transport, endosome to Golgi