Rabbit anti-Human, Mouse ATPBD3 Polyclonal Antibody | anti-CTU1 antibody

ATPBD3, ID (CTU1, ATPBD3, NCS6, Cytoplasmic tRNA 2-thiolation protein 1, ATP-binding domain-containing protein 3, Cancer-associated gene protein, Cytoplasmic tRNA adenylyltransferase 1)

Cat. Num. AAA6011632

• Gene Names: CTU1; NCS6; ATPBD3
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: ATPBD3; Polyclonal Antibody; ID (CTU1; NCS6; Cytoplasmic tRNA 2-thiolation protein 1; ATP-binding domain-containing protein 3; Cancer-associated gene protein; Cytoplasmic tRNA adenylyltransferase 1); Anti -ATPBD3; anti-CTU1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-CTU1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100
• Immunogen: ATPBD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 215-245 amino acids from the Central region of human ATPBD3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ATPBD3 Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the ATPBD3 antibody detected the ATPBD3 protein (arrow).)

Immunohistochemistry (IHC)

(ATPBD3 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATPBD3 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-CTU1 antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 119592390
• NCBI GeneID: 90353
• UniProt Accession #: Q7Z7A3; Q96GZ7; A8K558
• Molecular Weight: 36,450 Da
• NCBI Official Full Name: ATP binding domain 3
• NCBI Official Synonym Full Names: cytosolic thiouridylase subunit 1
• NCBI Official Symbol: CTU1
• NCBI Official Synonym Symbols: NCS6; ATPBD3
• NCBI Protein Information: cytoplasmic tRNA 2-thiolation protein 1; ATP binding domain 3; cancer-associated protein; cancer-associated gene protein; cytoplasmic tRNA adenylyltransferase 1; ATP-binding domain-containing protein 3; cytosolic thiouridylase subunit 1 homolog
• UniProt Protein Name: Cytoplasmic tRNA 2-thiolation protein 1
• Protein Family: Cytoplasmic tRNA 2-thiolation protein
• UniProt Gene Name: CTU1
• UniProt Synonym Gene Names: ATPBD3; NCS6
• UniProt Entry Name: CTU1_HUMAN

Uniprot Description

ATPBD3: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.

Protein type: EC 2.7.7.-

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: mitochondrion; cytosol

Molecular Function: protein binding; tRNA binding; nucleotidyltransferase activity

Biological Process: protein urmylation; tRNA wobble uridine modification