Western Blot (WB)
(ATPBD3 Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the ATPBD3 antibody detected the ATPBD3 protein (arrow).)
Rabbit anti-Human, Mouse ATPBD3 Polyclonal Antibody | anti-ATPBD3 antibody
ATPBD3, ID (CTU1, ATPBD3, NCS6, Cytoplasmic tRNA 2-thiolation protein 1, ATP-binding domain-containing protein 3, Cancer-associated gene protein, Cytoplasmic tRNA adenylyltransferase 1) (Azide free) (HRP)
Gene Names
CTU1; NCS6; ATPBD3
Reactivity
Human, Mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ATPBD3; Polyclonal Antibody; ID (CTU1; NCS6; Cytoplasmic tRNA 2-thiolation protein 1; ATP-binding domain-containing protein 3; Cancer-associated gene protein; Cytoplasmic tRNA adenylyltransferase 1) (Azide free) (HRP); CTU1; Cytoplasmic tRNA adenylyltransferase 1; anti-ATPBD3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-ATPBD3 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
ATPBD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 215-245 amino acids from the Central region of human ATPBD3.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(ATPBD3 Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the ATPBD3 antibody detected the ATPBD3 protein (arrow).)
Western Blot (WB)
(ATPBD3 Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the ATPBD3 antibody detected the ATPBD3 protein (arrow).)
Immunohistochemistry (IHC)
(ATPBD3 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATPBD3 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(ATPBD3 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATPBD3 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Product Categories/Family for anti-ATPBD3 antibody
References
Schlieker, C.D., et al. Proc. Natl. Acad. Sci. U.S.A. 105(47):18255-18260(2008). Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,450 Da
NCBI Official Full Name
cytoplasmic tRNA 2-thiolation protein 1
NCBI Official Synonym Full Names
cytosolic thiouridylase subunit 1
NCBI Official Symbol
CTU1
NCBI Official Synonym Symbols
NCS6; ATPBD3
NCBI Protein Information
cytoplasmic tRNA 2-thiolation protein 1; ATP binding domain 3; cancer-associated protein; cancer-associated gene protein; cytoplasmic tRNA adenylyltransferase 1; ATP-binding domain-containing protein 3; cytosolic thiouridylase subunit 1 homolog
UniProt Protein Name
Cytoplasmic tRNA 2-thiolation protein 1
UniProt Gene Name
CTU1
UniProt Synonym Gene Names
ATPBD3; NCS6
UniProt Entry Name
CTU1_HUMAN
Uniprot Description
ATPBD3: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
Protein type: EC 2.7.7.-
Chromosomal Location of Human Ortholog: 19q13.41
Cellular Component: mitochondrion; cytosol
Molecular Function: protein binding; tRNA binding; nucleotidyltransferase activity
Biological Process: tRNA wobble uridine modification; protein urmylation
Research Articles on ATPBD3
Similar Products
Product Notes
The ATPBD3 ctu1 (Catalog #AAA6276759) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATPBD3, ID (CTU1, ATPBD3, NCS6, Cytoplasmic tRNA 2-thiolation protein 1, ATP-binding domain-containing protein 3, Cancer-associated gene protein, Cytoplasmic tRNA adenylyltransferase 1) (Azide free) (HRP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ATPBD3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ATPBD3 ctu1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATPBD3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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