Testing Data #1
(Jurkat cells were cultured in the absence (healthy) or presence (dying) of staurosporine to induce apoptosis and then stained with TMRM dye. Cells were analyzed using a Nikon Eclipse E800 photomicroscope using a green excitation filter (510-560 nm) in tandem with a 570-620 nm emission filter. Apoptotic cells with depolarized mitochondria exhibit reduced orange/red fluorescence)
MITOCHONDRIAL MEMBRANE POTENTIAL MitoPT TMRM KIT Assay Kit
MITOCHONDRIAL MEMBRANE POTENTIAL KIT
Applications
Flow Cytometry, Functional Assay, Immunofluorescence
Synonyms
MITOCHONDRIAL MEMBRANE POTENTIAL MitoPT TMRM KIT; MITOCHONDRIAL MEMBRANE POTENTIAL KIT; MITOCHONDRIAL MEMBRANE POTENTIAL MitoPT TMRM KIT assay kit
Form/Format
Kit
Applicable Applications for MITOCHONDRIAL MEMBRANE POTENTIAL MitoPT TMRM KIT assay kit
Flow cytometry (FC/FACS), Immunofluorescence (IF)
Reagents In The Kits
MitoPTTM TMRM Reagent - lyophilized - to make up 1mM stock, 10X Assay Buffer, 125mL, 600uL vial 50mM Carbonylcyanide m - chlorophenylhydrazone (CCCP)
concentrate in DMSO.
concentrate in DMSO.
Preparation and Storage
Store the unopened kit (and each unopened component) at -20 degree C until the expiration date.
Protect the MitoPTTM TMRM reagent from light at all times.
Once reconstituted, the 1mM MitoPTTM TMRM stock should be stored at -20 degree C protected from light.
Shelf Life: The 1mM MitoPTTM TMRM reconstituted stock is stable for up to 12 months and may be thawed three times during that time.
Protect the MitoPTTM TMRM reagent from light at all times.
Once reconstituted, the 1mM MitoPTTM TMRM stock should be stored at -20 degree C protected from light.
Shelf Life: The 1mM MitoPTTM TMRM reconstituted stock is stable for up to 12 months and may be thawed three times during that time.
Testing Data #1
(Jurkat cells were cultured in the absence (healthy) or presence (dying) of staurosporine to induce apoptosis and then stained with TMRM dye. Cells were analyzed using a Nikon Eclipse E800 photomicroscope using a green excitation filter (510-560 nm) in tandem with a 570-620 nm emission filter. Apoptotic cells with depolarized mitochondria exhibit reduced orange/red fluorescence)
Testing Data #1
(Jurkat cells were cultured in the absence (healthy) or presence (dying) of staurosporine to induce apoptosis and then stained with TMRM dye. Cells were analyzed using a Nikon Eclipse E800 photomicroscope using a green excitation filter (510-560 nm) in tandem with a 570-620 nm emission filter. Apoptotic cells with depolarized mitochondria exhibit reduced orange/red fluorescence)
Related Product Information for MITOCHONDRIAL MEMBRANE POTENTIAL MitoPT TMRM KIT assay kit
Description: The MitoPTTM TMRM kit uses a quick and easy staining method to clearly differentiate between non-apoptotic and apoptotic cells through mitochondrial functionality.
Test Principle: An early indication of apoptosis involves a collapse in the electrochemical gradient across the mitochondrial membrane. Loss of mitochondrial membrane potential can be detected by a unique fluorescent cationic dye known as TMRM (tetramethylrhodamine methyl ester), that has been incorporated into the MitoPTTM TMRM kit.
The MitoPTTM TMRM reagent easily penetrates cells and enters the mitochondria. It aggregates in the mitochondria of non-apoptotic cells and fluoresces bright orange/red, whilst in apoptotic cells it diffuses throughout the cell. Once dispersed, the reagent assumes a monomeric form and exhibits a reduced orange/red fluorescence level. This allows an easy distinction between apoptotic and non-apoptotic fluorescent cells which can be read with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.
Test Principle: An early indication of apoptosis involves a collapse in the electrochemical gradient across the mitochondrial membrane. Loss of mitochondrial membrane potential can be detected by a unique fluorescent cationic dye known as TMRM (tetramethylrhodamine methyl ester), that has been incorporated into the MitoPTTM TMRM kit.
The MitoPTTM TMRM reagent easily penetrates cells and enters the mitochondria. It aggregates in the mitochondria of non-apoptotic cells and fluoresces bright orange/red, whilst in apoptotic cells it diffuses throughout the cell. Once dispersed, the reagent assumes a monomeric form and exhibits a reduced orange/red fluorescence level. This allows an easy distinction between apoptotic and non-apoptotic fluorescent cells which can be read with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.
Similar Products
Product Notes
The MITOCHONDRIAL MEMBRANE POTENTIAL MitoPT TMRM KIT (Catalog #AAA239183) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MITOCHONDRIAL MEMBRANE POTENTIAL MitoPT TMRM KIT can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS), Immunofluorescence (IF). Researchers should empirically determine the suitability of the MITOCHONDRIAL MEMBRANE POTENTIAL MitoPT TMRM KIT for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MITOCHONDRIAL MEMBRANE POTENTIAL MitoPT TMRM KIT, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
