Rabbit anti-Human, Rat LRRK1 Polyclonal Antibody | anti-LRRK1 antibody

LRRK1 (Leucine-rich Repeat Kinase 1, Leucine-rich Repeat Serine/threonine-protein Kinase 1, FLJ23119, FLJ27465, KIAA1790, RIPK6, Roco1)

Cat. Num. AAA6011102

• Gene Names: LRRK1; RIPK6; Roco1
• Reactivity: Human, Rat
• Applications: Western Blot, Immunoprecipitation
• Purity: Serum; Serum
• Synonyms: LRRK1; Polyclonal Antibody; LRRK1 (Leucine-rich Repeat Kinase 1; Leucine-rich Repeat Serine/threonine-protein Kinase 1; FLJ23119; FLJ27465; KIAA1790; RIPK6; Roco1); Anti -LRRK1 (Leucine-rich Repeat Kinase 1; anti-LRRK1 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LRRK1. Species Crossreactivity: rat.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid.
• Sequence: MSQRPPSMYWCVGPEESAVCPERAMETLNGAGDTGGKPSTRGGDPAARSRRTEGIRAAYRRGDRGGARDLLEEACDQCASQLEKGQLLSIPAAYGDLEMVRYLLSKRLVELPTEPTDDNPAVVAAYFGHTAVVQELLESLPGPCSPQRLLNWMLALACQRGHLGVVKLLVLTHGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIAIFLLRHGAYFCSYILLDSPDPSKHLLRKYFIEASPLPSSYPGKTVSSHCLWSVF

• Applicable Applications for anti-LRRK1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.
• Immunogen: Full length human LRRK1, aa1-261 (AAH68080.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(LRRK1 rabbit polyclonal antibody. Western Blot analysis of LRRK1 expression in rat muscle.)

Western Blot (WB)

(Western Blot analysis of LRRK1 expression in transfected 293T cell line by LRRK1 polyclonal antibody. Lane 1: LRRK1 transfected lysate (28.6kD). Lane 2: Non-transfected lysate.)

Immunoprecipitation (IP)

(Immunoprecipitation of LRRK1 transfected lysate using LRRK1 rabbit polyclonal antibody and Protein A Magnetic Bead and immunoblotted with LRRK1 purified mouse polyclonal antibody.)

Related Product Information for anti-LRRK1 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Product Categories/Family for anti-LRRK1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 90991702
• NCBI GeneID: 79705
• NCBI Accession #: NP_078928.3
• NCBI GenBank Nucleotide #: NM_024652.3
• UniProt Accession #: Q38SD2; Q6NVH5; Q6NYC0; Q6ZNL9; Q6ZNM9; Q96JN5; Q9H5S3
• Molecular Weight: 225,393 Da
• NCBI Official Full Name: leucine-rich repeat serine/threonine-protein kinase 1
• NCBI Official Synonym Full Names: leucine-rich repeat kinase 1
• NCBI Official Symbol: LRRK1
• NCBI Official Synonym Symbols: RIPK6; Roco1
• NCBI Protein Information: leucine-rich repeat serine/threonine-protein kinase 1
• UniProt Protein Name: Leucine-rich repeat serine/threonine-protein kinase 1
• Protein Family: Leucine-rich repeat serine/threonine-protein kinase
• UniProt Gene Name: LRRK1
• UniProt Synonym Gene Names: KIAA1790
• UniProt Entry Name: LRRK1_HUMAN

Uniprot Description

LRRK1: a large multidomain protein kinase with a TKL-type kinase domain, multiple protein-protein interaction domains and a mitochondrial Rho domain (MIRO). Involved in the trafficking of epidermal growth factor (EGFR) from early to late endosomes. Forms a complex with activated EGFR via the adaptor protein Grb2. Subsequently, LRRK1 and EGFR are internalized and co-localized in early endosomes. LRRK1 is phosphorylated by EGFR, inhibiting its kinase activity. The reduction of kinase activity plays an important role in endosomal trafficking of the EGFR. Serves as a scaffold expediting the interaction of EGFR with the endosomal sorting complex required for efficient sorting of EGFR to the inner vesicles of multivesicular bodies. Plays a critical role in negative regulation of bone mass in part through modulating the c-Src signaling pathway in mice. Phosphorylation of c-Src at Tyr-527 is significantly elevated whereas at Tyr-416 is decreased in LRRK1 KO mouse osteoclasts.

Protein type: Protein kinase, TKL; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; TKL group; LRRK family

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: mitochondrion; cytoplasm

Molecular Function: protein serine/threonine kinase activity; identical protein binding; protein binding; GTP binding; metal ion binding; ATP binding

Biological Process: negative regulation of peptidyl-tyrosine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; small GTPase mediated signal transduction; protein amino acid phosphorylation; bone resorption