anti-Human XRCC1 Monoclonal Antibody | anti-XRCC1 antibody
Anti - XRCC1 (X-Ray Repair Cross Complementing)
Gene Names
XRCC1; RCC
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Affinity purified over the control-peptide Sepharose
Synonyms
XRCC1; Monoclonal Antibody; Anti - XRCC1 (X-Ray Repair Cross Complementing); XRCC1 (X-Ray Repair Cross Complementing); anti-XRCC1 antibody
Host
Host: Mouse
Source: Human
Source: Human
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
3H56
Specificity
Recognizes human protein.
Purity/Purification
Affinity purified over the control-peptide Sepharose
Form/Format
Affinity Purified
Supplied as a liquid in 10 mM PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide, 40% glycerol.
Supplied as a liquid in 10 mM PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide, 40% glycerol.
Concentration
0.2mg/ml (varies by lot)
Sequence Length
633
Applicable Applications for anti-XRCC1 antibody
Western Blot, Immunoprecipitation
Application Notes
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Dilution: Western Blot: An initial concentration of 1-5ug/ml is recommended for Western. The antibody recognizes >70kD in HeLa cells and testis.
Immunoprecipitation: 5-10ug/mg of protein lysate:.
Optimal dilutions to be determined by the researcher.
Dilution: Western Blot: An initial concentration of 1-5ug/ml is recommended for Western. The antibody recognizes >70kD in HeLa cells and testis.
Immunoprecipitation: 5-10ug/mg of protein lysate:.
Optimal dilutions to be determined by the researcher.
Immunogen
Purified Human recombinant XRCC1 protein
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-XRCC1 antibody
X-Ray Repair Cross Complementing (XRCC1) plays a role in excision repair of DNA after ionizing irradiation. XRCC1 binds to DNA ligase III through the C-terminal 96 amino acids and to DNA polymerase beta through the N-terminal half.. In testis XRCC1 is expressed at high levels. Cells with a mutation of this gene exhibit decreased single strand break repair and reduced recombination repair. They show increased double strand breaks, and sister chromatid exchange is increased up to 10-fold. The XRCC1 might serve as a scaffold protein during base excision-repair.
Product Categories/Family for anti-XRCC1 antibody
References
• 1.Brookman KW et al (1994) Genomics 22, 180-188; Thompson LH et al (1990) Mol Cell Biol. 10, 6160-6171; Cappeli E et al (1997) J. Biol. Chem. 272, 23970-23975; Nash RA et al (1997) Biochemistry 36, 5207-5211.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
69,477 Da
NCBI Official Full Name
XRCC1
NCBI Official Synonym Full Names
X-ray repair complementing defective repair in Chinese hamster cells 1
NCBI Official Symbol
XRCC1
NCBI Official Synonym Symbols
RCC
NCBI Protein Information
DNA repair protein XRCC1; X-ray repair cross-complementing protein 1; X-ray-repair, complementing defective, repair in Chinese hamster
UniProt Protein Name
DNA repair protein XRCC1
Protein Family
UniProt Gene Name
XRCC1
UniProt Entry Name
XRCC1_HUMAN
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Product Notes
The XRCC1 xrcc1 (Catalog #AAA601072) is an Antibody produced from Host: Mouse Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti - XRCC1 (X-Ray Repair Cross Complementing) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's XRCC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot, Immunoprecipitation. Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Dilution: Western Blot: An initial concentration of 1-5ug/ml is recommended for Western. The antibody recognizes >70kD in HeLa cells and testis. Immunoprecipitation: 5-10ug/mg of protein lysate:. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the XRCC1 xrcc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "XRCC1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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