A Disintegrin And Metalloproteinase With Thrombospondin 5 Recombinant Protein | ADAMTS5 recombinant protein

Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5)

Cat. Num. AAA2029471

• Gene Names: ADAMTS5; ADMP-2; ADAM-TS5; ADAMTS-5; ADAMTS11; ADAM-TS 5; ADAMTS-11; ADAM-TS 11
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: A Disintegrin And Metalloproteinase With Thrombospondin 5; Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5); ADAMTS5 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 9.3 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ELPGQT YDATQQCNLT FGPEYSVCPG MDVCARLWCA VVRQGQMVCL TKKLPAVEGT PCGKGRICLQ GKCVDKTKKK YYSTSSHGNW GSWGSWGQCS RSCGGGVQFA YRHCNNPAPRNNGRYCTGKR AIYRSCSLMP CP
• Sequence Length: 930

• Applicable Applications for ADAMTS5 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 19.0kDa
• Accurate Molecular Mass (KD): 19kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Glu485~Pro622
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 195539372
• NCBI GeneID: 11096
• NCBI Accession #: NP_008969.2
• NCBI GenBank Nucleotide #: NM_007038.3
• Molecular Weight: 101,718 Da
• NCBI Official Full Name: A disintegrin and metalloproteinase with thrombospondin motifs 5 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase with thrombospondin type 1 motif, 5
• NCBI Official Symbol: ADAMTS5
• NCBI Official Synonym Symbols: ADMP-2; ADAM-TS5; ADAMTS-5; ADAMTS11; ADAM-TS 5; ADAMTS-11; ADAM-TS 11
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 5
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 5
• Protein Family: A disintegrin and metalloproteinase with thrombospondin motifs
• UniProt Gene Name: ADAMTS5
• UniProt Synonym Gene Names: ADAMTS11; ADMP2; ADAM-TS 5; ADAM-TS5; ADAMTS-5; ADAM-TS 11; ADAMTS-11
• UniProt Entry Name: ATS5_HUMAN

NCBI Description

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage. [provided by RefSeq, Jul 2008]

Uniprot Description

ADAMTS5: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period.

Protein type: Protease; Secreted, signal peptide; Extracellular matrix; Motility/polarity/chemotaxis; Secreted; EC 3.4.24.-

Chromosomal Location of Human Ortholog: 21q21.3

Cellular Component: proteinaceous extracellular matrix; endoplasmic reticulum lumen; extracellular region

Molecular Function: integrin binding; protein binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity

Biological Process: protein amino acid O-linked glycosylation; extracellular matrix disassembly; extracellular matrix organization and biogenesis; cellular protein metabolic process; defense response to bacterium; proteolysis; post-translational protein modification