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Western Blot (WB)
(DARS Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the DARS antibody detected the DARS protein (arrow).)
Rabbit anti-Human DARS Polyclonal Antibody
DARS, NT (DARS, Aspartate-tRNA ligase, cytoplasmic, Aspartyl-tRNA synthetase, Cell proliferation-inducing gene 40 protein)
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
PurifiedPurified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
DARS; Polyclonal Antibody; NT (DARS; Aspartate-tRNA ligase; cytoplasmic; Aspartyl-tRNA synthetase; Cell proliferation-inducing gene 40 protein); Anti -DARS; anti-DARS antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Purified
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-DARS antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:50-100
Immunohistochemistry: 1:50-100
Immunofluorescence: 1:10-50
Flow Cytometry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:50-100
Immunohistochemistry: 1:50-100
Immunofluorescence: 1:10-50
Flow Cytometry: 1:10-50
Immunogen
DARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-190 amino acids from the N-terminal region of human DARS.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(DARS Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the DARS antibody detected the DARS protein (arrow).)
Western Blot (WB)
(DARS Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the DARS antibody detected the DARS protein (arrow).)
Western Blot (WB)
(Western blot analysis of DARS (arrow) using rabbit polyclonal DARS Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DARS gene.)
Western Blot (WB)
(Western blot analysis of DARS (arrow) using rabbit polyclonal DARS Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DARS gene.)
Immunohistochemistry (IHC)
(DARS Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DARS Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(DARS Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DARS Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of DARS Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of DARS Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of DARS Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of DARS Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Related Product Information for anti-DARS antibody
Aspartyl-tRNA synthetase (DARS) is part of a multienzyme complex of aminoacyl-tRNA synthetases. Aspartyl-tRNA synthetase charges its cognate tRNA with aspartate during protein biosynthesis.
Product Categories/Family for anti-DARS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
DarS
Similar Products
Product Notes
The DARS (Catalog #AAA6010606) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DARS, NT (DARS, Aspartate-tRNA ligase, cytoplasmic, Aspartyl-tRNA synthetase, Cell proliferation-inducing gene 40 protein) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DARS can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:50-100 Immunohistochemistry: 1:50-100 Immunofluorescence: 1:10-50 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the DARS for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DARS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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