Rabbit anti-Human RGS3 Polyclonal Antibody | anti-RGS3 antibody

RGS3 Antibody (N-Term)

Cat. Num. AAA9216496

• Gene Names: RGS3; C2PA; RGP3
• Reactivity: Human
• Applications: Western Blot
• Synonyms: RGS3; Polyclonal Antibody; RGS3 Antibody (N-Term); Purified Rabbit Polyclonal Antibody (Pab); Regulator of G-protein signaling 3; RGP3; anti-RGS3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This RGS3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 273-307 amino acids from human RGS3.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 519

• Applicable Applications for anti-RGS3 antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:1000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-RGS3 Antibody (N-Term) at 1:1000 dilutionLane 1: MCF-7 whole cell lysateLane 2: U-87 MG whole cell lysateLane 3: DU145 whole cell lysateLane 4: Hela whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 57 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-RGS3 antibody

Down-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G- protein-mediated release of inositol phosphates and activation of MAP kinases.

Product Categories/Family for anti-RGS3 antibody

Signal Transduction

References

Druey K.M.,et al.Nature 379:742-746(1996). Kehrl J.H.,et al.Genomics 79:860-868(2002). Ota T.,et al.Nat. Genet. 36:40-45(2004). Chatterjee T.K.,et al.Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases. Puhl H.L. III,et al.Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 442654297
• NCBI GeneID: 5998
• NCBI Accession #: NP_001263189.1
• NCBI GenBank Nucleotide #: NM_001276260.1
• UniProt Accession #: P49796; Q5VXB8; Q5VXC1; Q5VZ05; Q5VZ06; Q6ZRM5; Q8IUQ1; Q8NC47; Q8NFN4; A6NHA0; A8K0V1; B3KUB2
• Molecular Weight: 132336
• NCBI Official Full Name: regulator of G-protein signaling 3 isoform 2
• NCBI Official Synonym Full Names: regulator of G-protein signaling 3
• NCBI Official Symbol: RGS3
• NCBI Official Synonym Symbols: C2PA; RGP3
• NCBI Protein Information: regulator of G-protein signaling 3
• UniProt Protein Name: Regulator of G-protein signaling 3
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS3
• UniProt Synonym Gene Names: RGP3; RGS3
• UniProt Entry Name: RGS3_HUMAN

NCBI Description

This gene encodes a member of the regulator of G-protein signaling (RGS) family. This protein is a GTPase-activating protein that inhibits G-protein-mediated signal transduction. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. Long isoforms are largely cytosolic and plasma membrane-associated with a function in Wnt signaling and in the epithelial mesenchymal transition, while shorter N-terminally-truncated isoforms can be nuclear. [provided by RefSeq, Jan 2013]

Uniprot Description

RGS3: Down-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G- protein-mediated release of inositol phosphates and activation of MAP kinases. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs; GAPs, RGS

Chromosomal Location of Human Ortholog: 9q32

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; GTPase activator activity

Biological Process: regulation of G-protein coupled receptor protein signaling pathway; inactivation of MAPK activity; positive regulation of GTPase activity