Rabbit anti-Human Connexin 45 Polyclonal Antibody | anti-Gjc1 antibody

Connexin 45, NT (Gap Junction gamma-1 Protein, Gap Junction alpha-7 Protein, GJA7, Connexin-45, Cx45, GJC1)

Cat. Num. AAA6009746

• Gene Names: Gjc1; Cx45; Gja7; Cnx45; Gja-7; C130009G16Rik
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Connexin 45; Polyclonal Antibody; NT (Gap Junction gamma-1 Protein; Gap Junction alpha-7 Protein; GJA7; Connexin-45; Cx45; GJC1); Anti -Connexin 45; anti-Gjc1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GJA7.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-Gjc1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry (paraffin): 1:50-1:100
• Immunogen: Synthetic peptide selected from the N-terminal region of human GJA7 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of hGJA7-H104.Connexin in MDA-MB435 cell line lysates (35ug/lane).GJA7 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-Gjc1 antibody

Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell closely packed transmembrane channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. Connexins are designated by their molecular mass. Another system of nomenclature divides gap junction proteins into 2 categories, alpha and beta, according to sequence similarities at the nucleotide and amino acid levels. For example, CX43 is designated alpha-1 gap junction protein, whereas CX32 and CX26 are called beta-1 and beta-2 gap junction proteins, respectively. This nomenclature emphasizes that CX32 and CX26 are more homologous to each other than either of them is to CX43. Connexins have four transmembrane, three intracellular, and two extracellular regions. Different tissues express different connexins, though tissue specificities overlap, and a given tissue or cell can express several different connexins. Developmental regulation of at least some of the connexin genes has been found. Embryo implantation is regulated in part by temporally changing patterns of expression of connexins in the embryo and the maternal decidua.

Product Categories/Family for anti-Gjc1 antibody

Antibodies; Abs to Connexin; Junction Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 12188899
• NCBI GeneID: 14615
• UniProt Accession #: P28229
• Molecular Weight: 45,666 Da
• NCBI Official Full Name: connexin 45
• NCBI Official Synonym Full Names: gap junction protein, gamma 1
• NCBI Official Symbol: Gjc1
• NCBI Official Synonym Symbols: Cx45; Gja7; Cnx45; Gja-7; C130009G16Rik
• NCBI Protein Information: gap junction gamma-1 protein; connexin 45; connexin-45; gap junction alpha-7 protein; gap junction membrane channel protein alpha 7
• UniProt Protein Name: Gap junction gamma-1 protein
• Protein Family: Gap junction gamma-1 protein
• UniProt Gene Name: Gjc1
• UniProt Synonym Gene Names: Cxn-45; Gja7; Cx45
• UniProt Entry Name: CXG1_MOUSE

Uniprot Description

GJC1: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Belongs to the connexin family. Gamma-type subfamily.

Protein type: Membrane protein, integral; Cell adhesion; Membrane protein, multi-pass

Cellular Component: connexon complex; membrane; integral to membrane; plasma membrane; gap junction; cell junction

Molecular Function: protein binding; ion channel activity; gap junction channel activity

Biological Process: cardiac muscle development; synaptic transmission; visual perception; cell-cell signaling; heart development; cell communication; vasculogenesis; transmembrane transport; cell development