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Immunohistochemistry (IHC) (Human Tonsil (formalin-fixed, paraffin-embedded) stained with CENTB1 antibody at 5ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Goat anti-Human CENTB1 Polyclonal Antibody | anti-ACAP1 antibody

CENTB1, CT (Arf-GAP with Coiled-coil, ANK Repeat and PH Domain-containing Protein 1, Centaurin-beta-1, Cnt-b1, ACAP1, KIAA0050)

Gene Names
ACAP1; CENTB1
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Synonyms
CENTB1; Polyclonal Antibody; CT (Arf-GAP with Coiled-coil; ANK Repeat and PH Domain-containing Protein 1; Centaurin-beta-1; Cnt-b1; ACAP1; KIAA0050); Anti -CENTB1; anti-ACAP1 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes human CENTB1. Species sequence homology: mouse and rat
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Applicable Applications for anti-ACAP1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: ELISA: 1:32,000
Western Blot: 1:32,000 & 0.5-2ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Immunogen
Synthetic peptide corresponding to C-EKLSRRSHDLHTL from the C-terminal of human CENTB1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Tonsil (formalin-fixed, paraffin-embedded) stained with CENTB1 antibody at 5ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Immunohistochemistry (IHC) (Human Tonsil (formalin-fixed, paraffin-embedded) stained with CENTB1 antibody at 5ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Western Blot (WB)

(Antibody2 staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

Western Blot (WB) (Antibody2 staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Related Product Information for anti-ACAP1 antibody
Centaurin beta 1 and Centaurin beta 2 are recruited to platelet-derived growth factor-induced dorsal membrane ruffles in NIH 3T3 mouse fibroblasts, and overexpression inhibited ruffle formation. In vitro, Centaurin beta 1 and Centaurin beta 2 preferred ARF6 as substrate rather than ARF1 or ARF5, and the GAP activity of both Centaurins was dependent upon phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). The isolated PH domain of mouse Centaurin beta 2 exhibits moderate affinity for PtdIns(3,5)P2, but it does not bind any other phosphoinositides tested, including PtdIns(4,5)P2. Mutation of a highly conserved arginine in both Centaurins result in lack of ARF-GAP activity. Overexpression in HeLa cells of either Centaurin blocks the formation of ARF6-dependent protrusions. Both Centaurins are also recruited to peripheral, tubular membranes, where activation of ARF6 occurs and allows membrane recycling back to the plasma membrane.
Product Categories/Family for anti-ACAP1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
81,536 Da
NCBI Official Full Name
centaurin, beta 1, isoform CRA_b
NCBI Official Synonym Full Names
ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
NCBI Official Symbol
ACAP1
NCBI Official Synonym Symbols
CENTB1
NCBI Protein Information
arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1; cnt-b1; centaurin-beta-1; centaurin, beta 1; Arf GAP with coiled coil, ANK repeat and PH domains 1
UniProt Protein Name
Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1
UniProt Gene Name
ACAP1
UniProt Synonym Gene Names
CENTB1; KIAA0050; Cnt-b1
UniProt Entry Name
ACAP1_HUMAN

Uniprot Description

CENTB1: a member of the centaurin family of ADP-ribosylation factor directed GTPase-activating proteins (GAPs). A GAP for ARF1 and ARF5. Two alternatively-spliced isoforms have been described. Isoform 1 is brain-specific, and isoform 2 is ubiquitously expressed, with highest levels in brain and heart. Isoform 1 participates to the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. Regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. The PH domains of isoforms 1 and 2 bind phospholipids, but they differentially regulate subcellular location. The PH domain of isoform 1 directs the protein to the nucleus, but the PH domain of isoform 2 directs it to the cytosol.

Protein type: GAPs, ARF; Motility/polarity/chemotaxis; GAPs

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: membrane; recycling endosome membrane

Molecular Function: zinc ion binding

Biological Process: protein transport; positive regulation of GTPase activity

Research Articles on ACAP1

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Product Notes

The ACAP1 acap1 (Catalog #AAA6009465) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The CENTB1, CT (Arf-GAP with Coiled-coil, ANK Repeat and PH Domain-containing Protein 1, Centaurin-beta-1, Cnt-b1, ACAP1, KIAA0050) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CENTB1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot and Immunohistochemistry. Dilution: ELISA: 1:32,000 Western Blot: 1:32,000 & 0.5-2ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml. Researchers should empirically determine the suitability of the ACAP1 acap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CENTB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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