Rabbit anti-Human, Mouse ARK5 Polyclonal Antibody

ARK5, CT (NUAK Family SNF1-like Kinase 1, AMPK-related Protein Kinase 5, KIAA0537, NUAK1)

Cat. Num. AAA6008162

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: ARK5; Polyclonal Antibody; CT (NUAK Family SNF1-like Kinase 1; AMPK-related Protein Kinase 5; KIAA0537; NUAK1); Anti -ARK5; anti-ARK5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ARK5. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-ARK5 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human ARK5 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of mouse brain tissue lysate using MBS6008162. ARK5 (arrow) was detected using MBS6008162. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Immunohistochemistry of formalin-fixed and paraffin-embedded human cancer tissue using MBS6008162, followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of MBS6008162 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-ARK5 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Product Categories/Family for anti-ARK5 antibody

Antibodies; Abs to Protein Kinases