Rabbit anti-Human, Mouse AR Polyclonal Antibody | anti-AR antibody

Phospho-AR (Ser650) Antibody

Cat. Num. AAA7133286

• Gene Names: AR; KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
• Synonyms: AR; Polyclonal Antibody; Phospho-AR (Ser650) Antibody; ANDR; DHTR; anti-AR antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
• Form/Format: Liquid; Supplied at 1.0mg/mL In Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol.

• Applicable Applications for anti-AR antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:100
• Immunogen: Peptide sequence around phosphorylation site of serine 650 (T-T-S(p)-P-T) derived from Human Androgen Receptor.
• Immunogen Species: Human
• Conjugate: Non-conjugated
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Androgen Receptor (Phospho-Ser650) Antibody (left) or the same antibody preincubated with blocking peptide (right).)

Western Blot (WB)

(Western blot analysis of extracts from 293 cells, treated with PMA or calf intestinal phosphatase (CIP), using Androgen Receptor (Phospho-Ser650) Antibody.)

Product Categories/Family for anti-AR antibody

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 21322252
• NCBI GeneID: 367
• NCBI Accession #: NP_000035.2
• NCBI GenBank Nucleotide #: NM_000044.3
• UniProt Accession #: P10275; Q9UD95; A2RUN2; B1AKD7
• Molecular Weight: 98,989 Da
• NCBI Official Full Name: androgen receptor isoform 1
• NCBI Official Synonym Full Names: androgen receptor
• NCBI Official Symbol: AR
• NCBI Official Synonym Symbols: KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
• NCBI Protein Information: androgen receptor; dihydrotestosterone receptor; androgen nuclear receptor variant 2; nuclear receptor subfamily 3 group C member 4
• UniProt Protein Name: Androgen receptor
• Protein Family: Arpin
• UniProt Gene Name: AR
• UniProt Synonym Gene Names: DHTR; NR3C4
• UniProt Entry Name: ANDR_HUMAN

NCBI Description

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

AR: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.

Protein type: Nuclear receptor; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: Xq12

Cellular Component: nucleoplasm; protein complex; nuclear chromatin; cytoplasm; nucleus

Molecular Function: protein dimerization activity; ligand-dependent nuclear receptor activity; zinc ion binding; androgen binding; beta-catenin binding; transcription factor binding; protein binding; androgen receptor activity; enzyme binding; DNA binding; chromatin binding; transcription factor activity; receptor binding

Biological Process: prostate gland development; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; signal transduction; protein oligomerization; activation of NF-kappaB transcription factor; negative regulation of integrin biosynthetic process; cell proliferation; cell-cell signaling; transport; positive regulation of cell proliferation; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase III promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; positive regulation of integrin biosynthetic process; cell growth; sex differentiation; positive regulation of phosphorylation

Disease: Androgen Insensitivity Syndrome; Prostate Cancer; Androgen Insensitivity, Partial; Hypospadias 1, X-linked; Spinal And Bulbar Muscular Atrophy, X-linked 1