Western Blot (WB)
(Western Blot analysis of mouse NIH-3T3 cell line lysates (35ug/lane) using MBS6007719. This demonstrates that MBS6007719 detected the DAG1 protein (arrow).)
Rabbit anti-Human, Mouse DAG1 Polyclonal Antibody | anti-dag1 antibody
DAG1, CT (DAG1, Dystroglycan, Dystrophin-associated glycoprotein 1, Alpha-dystroglycan, Beta-dystroglycan)
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified by Protein A affinity chromatography.
Synonyms
DAG1; Polyclonal Antibody; CT (DAG1; Dystroglycan; Dystrophin-associated glycoprotein 1; Alpha-dystroglycan; Beta-dystroglycan); Anti -DAG1; anti-dag1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes Human DAG1
Purity/Purification
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Concentration
0.61 mg/ml (varies by lot)
Applicable Applications for anti-dag1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Dilution:
Western Blot: 1:1000
Immunohistochemistry (FFPE): 1:10-50
Western Blot: 1:1000
Immunohistochemistry (FFPE): 1:10-50
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 718-747 in the C-terminal region of human DAG1
Grade
Affinity Purified
Species Sequence Homology
Porcine and Rabbit
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of mouse NIH-3T3 cell line lysates (35ug/lane) using MBS6007719. This demonstrates that MBS6007719 detected the DAG1 protein (arrow).)
Western Blot (WB)
(Western Blot analysis of mouse NIH-3T3 cell line lysates (35ug/lane) using MBS6007719. This demonstrates that MBS6007719 detected the DAG1 protein (arrow).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of formalin fixed and paraffin embedded human skeletal muscle using MBS6007719 followed by peroxidase conjugation of the secondary antibody and DAB staining)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of formalin fixed and paraffin embedded human skeletal muscle using MBS6007719 followed by peroxidase conjugation of the secondary antibody and DAB staining)
Related Product Information for anti-dag1 antibody
Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants all encoding the same protein.
Product Categories/Family for anti-dag1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dystroglycan 1
NCBI Official Symbol
dag1
NCBI Protein Information
dystroglycan 1
Protein Family
Research Articles on dag1
Similar Products
Product Notes
The dag1 (Catalog #AAA6007719) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DAG1, CT (DAG1, Dystroglycan, Dystrophin-associated glycoprotein 1, Alpha-dystroglycan, Beta-dystroglycan) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DAG1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Dilution: Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:10-50. Researchers should empirically determine the suitability of the dag1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DAG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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