Western Blot (WB)
(DAG1 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the DAG1 antibody detected the DAG1 protein (arrow).)
Rabbit DAG1 Polyclonal Antibody | anti-DAG1 antibody
DAG1 Antibody (C-term)
Western Blot (WB)
(DAG1 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the DAG1 antibody detected the DAG1 protein (arrow).)
Western Blot (WB)
(DAG1 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the DAG1 antibody detected the DAG1 protein (arrow).)
Immunohistochemistry (IHC)
(DAG1 Antibody (C-term) (MBS9205082)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DAG1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(DAG1 Antibody (C-term) (MBS9205082)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DAG1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
dystrophin-glycoprotein complex which provides a linkage between
the subsarcolemmal cytoskeleton and the extracellular matrix.
Dystroglycan 1 is a candidate gene for the site of the mutation in
autosomal recessive muscular dystrophies. The dramatic reduction of
dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of
linkage between the sarcolemma and extracellular matrix, rendering
muscle fibers more susceptible to necrosis. Dystroglycan also
functions as dual receptor for agrin and laminin-2 in the Schwann
cell membrane. The muscle and nonmuscle isoforms of dystroglycan
differ by carbohydrate moieties but not protein sequence.
Alternative splicing results in multiple transcript variants all
encoding the same protein.
Nilsson, J., et al. Glycobiology 20(9):1160-1169(2010)
Lara-Chacon, B., et al. J. Cell. Biochem. 110(3):706-717(2010)
Sgambato, A., et al. Pathology 42(3):248-254(2010)
Masaki, T., et al. J. Biomed. Biotechnol. 2010, 740403 (2010) :
NCBI and Uniprot Product Information
NCBI Description
Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants all encoding the same protein.[provided by RefSeq, Apr 2010]
Uniprot Description
DAG1: a cytoskeletal protein that functions as a laminin receptor. Provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. May be involved in autosomal recessive muscular dystrophies. Its dramatic reduction in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. Binds to several types of arenaviruses. Is a target for the entry of Mycobacterium leprae into peripheral nerve Schwann cells. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence.
Protein type: Membrane protein, integral; Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 3p21
Cellular Component: dystroglycan complex; extracellular space; costamere; focal adhesion; basolateral plasma membrane; extracellular region; integral to membrane; lipid raft; nucleoplasm; dystrophin-associated glycoprotein complex; postsynaptic membrane; cytoskeleton; cell-cell adherens junction; lamellipodium; cytoplasm; plasma membrane; basement membrane; sarcolemma; filopodium
Molecular Function: tubulin binding; laminin-1 binding; viral receptor activity; protein binding; structural constituent of muscle; protein complex binding; calcium ion binding; SH2 domain binding; alpha-actinin binding; actin binding; vinculin binding
Biological Process: response to peptide hormone stimulus; nerve maturation; entry of virus into host cell; extracellular matrix organization and biogenesis; negative regulation of protein kinase B signaling cascade; cytoskeletal anchoring; negative regulation of MAPKKK cascade; membrane protein ectodomain proteolysis; NLS-bearing substrate import into nucleus; myelination in the peripheral nervous system; virus-host interaction; morphogenesis of an epithelial sheet; calcium-dependent cell-matrix adhesion; negative regulation of cell migration
Disease: Muscular Dystrophy-dystroglycanopathy (limb-girdle), Type C, 9; Muscular Dystrophy-dystroglycanopathy (congenital With Brain And Eye Anomalies), Type A, 9
Research Articles on DAG1
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Product Notes
The DAG1 dag1 (Catalog #AAA9205082) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 718-747. The DAG1 Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Pig, Rabbit) and may cross-react with other species as described in the data sheet. AAA Biotech's DAG1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the DAG1 dag1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DAG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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