Rabbit anti-Mouse, Rat Torc1 Monoclonal Antibody | anti-CRTC1 antibody

Torc1 (Transducer of Regulated CREB Activity 1)

Cat. Num. AAA600720

• Gene Names: CRTC1; MECT1; TORC1; TORC-1; WAMTP1
• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunoprecipitation
• Purity: As reported
• Synonyms: Torc1; Monoclonal Antibody; Torc1 (Transducer of Regulated CREB Activity 1); Anti -Torc1 (Transducer of Regulated CREB Activity 1); anti-CRTC1 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 8A39
• Specificity: Detects endogenous levels of total Torc1 protein.
• Purity/Purification: As reported
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/mlBSA, 50% glycerol, 0.02% sodium azide.

• Applicable Applications for anti-CRTC1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:50
• Immunogen: Synthetic peptide (KLH-coupled) derived from the sequence around Val463 of human Torc1.
• Preparation and Storage: For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of extracts from mouse and rat brain using MBS600720.)

Related Product Information for anti-CRTC1 antibody

Glucose homeostasis is regulated by hormones and cellular energy status. Elevations of blood glucose during feeding stimulate insulin release from pancreatic -cells through a glucose sensing pathway. Feeding also stimulates release of gut hormones such as glucagon-like peptide-1 (GLP-1), which further induces insulin release, inhibits glucagon release and promotes -cell viability. CREB-dependent transcription likely plays a role in both glucose sensing and GLP-1 signaling (1). The protein Torc2 (transducer of regulated CREB activity 2) functions as a CREB co-activator (2,3) and is implicated in mediating the effects of these two pathways (4). In quiescent cells, Torc2 is phosphorylated at Ser171 and becomes sequestered in the cytoplasm via an interaction with 14-3-3 proteins. Glucose and gut hormones lead to the dephosphorylation of Torc2 and its dissociation from 14-3-3 proteins. Dephosphorylated Torc2 enters the nucleus to promote CREB-dependent transcription. Torc2 plays a key role in the regulation of hepatic gluconeogenic gene transcription in response to hormonal and energy signals during fasting (5). Torc1 and Torc3, a pair of Torc2-related proteins, also act as CREB coactivators (2,3). Torc1, Torc2 and Torc3 associate with the HTLV Tax protein to promote Tax-dependent transcription of HTLV-1 long terminal repeats (6,7).

Product Categories/Family for anti-CRTC1 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 37693041
• NCBI GeneID: 23373
• UniProt Accession #: Q6UUV9; O75114; Q6Y3A3; Q7LDZ2; Q8IUL3; Q8IZ34; Q8IZL1; Q8N6W3; Q96AI8; Q9H801; A6NMG5
• Molecular Weight: 67,300 Da
• NCBI Official Full Name: transducer of regulated CREB protein 1
• NCBI Official Synonym Full Names: CREB regulated transcription coactivator 1
• NCBI Official Symbol: CRTC1
• NCBI Official Synonym Symbols: MECT1; TORC1; TORC-1; WAMTP1
• NCBI Protein Information: CREB-regulated transcription coactivator 1; mucoepidermoid carcinoma translocated protein 1; transducer of regulated cAMP response element-binding protein (CREB) 1
• UniProt Protein Name: CREB-regulated transcription coactivator 1
• Protein Family: CREB-regulated transcription coactivator
• UniProt Gene Name: CRTC1
• UniProt Synonym Gene Names: KIAA0616; MECT1; TORC1; WAMTP1; TORC-1
• UniProt Entry Name: CRTC1_HUMAN

Uniprot Description

TORC1: Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). In the hippocampus, involved in late-phase long- term potentiation (L-LTP) maintenance at the Schaffer collateral- CA1 synapses. May be required for dendritic growth of developing cortical neurons. Binds, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1. 'Arg-314' in the bZIP domain of CREB1 is essential for this interaction. Interaction, via its C-terminal, with TAF4, enhances recruitment of TAF4 to CREB1. Binds HTLV1 Tax. Highly expressed in adult and fetal brain. Located to specific regions such as the prefrontal cortex and cerebellum. Very low expression in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney. Belongs to the TORC family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; cAMP response element binding protein binding

Biological Process: transcription, DNA-dependent; viral reproduction; membrane hyperpolarization; activation of CREB transcription factor; rhythmic process; positive regulation of transcription from RNA polymerase II promoter; memory; protein homotetramerization; entrainment of circadian clock by photoperiod