Rabbit anti-Mouse Torc1 Polyclonal Antibody | anti-CRTC1 antibody

Torc1 (Transducer of Regulated CREB Activity 1)

Cat. Num. AAA612570

• Gene Names: CRTC1; MECT1; TORC1; TORC-1; WAMTP1
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by protein A affinityt chromatography.
• Synonyms: Torc1; Polyclonal Antibody; Torc1 (Transducer of Regulated CREB Activity 1); Anti -Torc1 (Transducer of Regulated CREB Activity 1); anti-CRTC1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: Detects endogenous levels of total Torc1 protein. Species sequence homology: Human - 100%.
• Purity/Purification: Affinity Purified; Purified by protein A affinityt chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol.

• Applicable Applications for anti-CRTC1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western blot 1:1000
• Immunogen: Synthetic peptide (KLH-coupled) derived from the sequence of human Torc1 (around G296).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of mouse brain extract using TORC1/CRTC1 MBS612570.)

Related Product Information for anti-CRTC1 antibody

Glucose homeostasis is regulated by hormones and cellular energy status. Elevations of blood glucose during feeding stimulate insulin release from pancreatic beta-cells through a glucose sensing pathway. Feeding also stimulates the release of gut hormones such as glucogon-like peptide-1 (GLP-1), which induces insulin release, inhibits glucagon release and promotes beta-cell viability. Studies suggest a role for CREB-dependent transcription in glucose sensing and GLP-1 signaling (1). Furthermore, Torc2 (transducer of regulated CREB activity 2), a co-activator of CREB (2,3), is implicated in mediating the effects of these two pathways (4). In quiescent cells, Torc2 is phosphorylated at Ser171 and is consequently sequestered in the cytoplasm via its interaction with 14-3-3 proteins. Glucose and gut hormones lead to the dephosphorylation of Torc2 and its subsequent dissociation with 14-3-3 proteins. Dephosphorylated Torc2 then enters into the nucleus and promotes CREB-dependent transcription. Torc2 is also shown to play a key role in the regulation of hepatic gluconeogenic gene transcription in response to hormonal and energy signals during fasting (5). In addition, two Torc2-related proteins, Torc1 and Torc3, have been identified (2,3). Both Torc1 and Torc3 are also CREB co-activators (3). Torc1, Torc2 and Torc3 have been shown to associate with Tax directly and promote Tax-dependent transcription of human T-cell leukemia virus type I long terminal repeats (6,7).

Product Categories/Family for anti-CRTC1 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 37693041
• NCBI GeneID: 23373
• UniProt Accession #: Q6UUV9; O75114; Q6Y3A3; Q7LDZ2; Q8IUL3; Q8IZ34; Q8IZL1; Q8N6W3; Q96AI8; Q9H801; A6NMG5
• Molecular Weight: 67,300 Da
• NCBI Official Full Name: transducer of regulated CREB protein 1
• NCBI Official Synonym Full Names: CREB regulated transcription coactivator 1
• NCBI Official Symbol: CRTC1
• NCBI Official Synonym Symbols: MECT1; TORC1; TORC-1; WAMTP1
• NCBI Protein Information: CREB-regulated transcription coactivator 1; mucoepidermoid carcinoma translocated protein 1; transducer of regulated cAMP response element-binding protein (CREB) 1
• UniProt Protein Name: CREB-regulated transcription coactivator 1
• Protein Family: CREB-regulated transcription coactivator
• UniProt Gene Name: CRTC1
• UniProt Synonym Gene Names: KIAA0616; MECT1; TORC1; WAMTP1; TORC-1
• UniProt Entry Name: CRTC1_HUMAN

Uniprot Description

TORC1: Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). In the hippocampus, involved in late-phase long- term potentiation (L-LTP) maintenance at the Schaffer collateral- CA1 synapses. May be required for dendritic growth of developing cortical neurons. Binds, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1. 'Arg-314' in the bZIP domain of CREB1 is essential for this interaction. Interaction, via its C-terminal, with TAF4, enhances recruitment of TAF4 to CREB1. Binds HTLV1 Tax. Highly expressed in adult and fetal brain. Located to specific regions such as the prefrontal cortex and cerebellum. Very low expression in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney. Belongs to the TORC family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; cAMP response element binding protein binding

Biological Process: transcription, DNA-dependent; viral reproduction; membrane hyperpolarization; activation of CREB transcription factor; rhythmic process; positive regulation of transcription from RNA polymerase II promoter; memory; protein homotetramerization; entrainment of circadian clock by photoperiod