Rabbit anti-Human APG5, cleaved Polyclonal Antibody | anti-APG5 antibody

APG5, cleaved (T193) (APG5 Autophagy 5-like, APG5-like, APG5L, Apoptosis-specific Protein, ASP, ATG5 Autophagy-related 5 Homolog, Autophagy Protein 5, hAPG5, Homolog of S Cerevisiae Autophagy 5)

Cat. Num. AAA6006552

• Gene Names: APG5; ATATG5; ATG5; AUTOPHAGY 5; MKP11.20; MKP11_20
• Reactivity: Human
• Applications: ELISA, Gel Super Shift Assay
• Purity: Affinity Purified; Purified by peptide immunoaffinity chromatography.
• Synonyms: APG5; cleaved; Polyclonal Antibody; cleaved (T193) (APG5 Autophagy 5-like; APG5-like; APG5L; Apoptosis-specific Protein; ASP; ATG5 Autophagy-related 5 Homolog; Autophagy Protein 5; hAPG5; Homolog of S Cerevisiae Autophagy 5); Anti -APG5; anti-APG5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by peptide immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-APG5 antibody: ELISA (EL/EIA), Gel Shift Assay (GS/EMSA)
• Application Notes: Suitable for use in Dot Blot, ELISA; Dilution: ELISA1:1,000; Dot blot 1:100-500
• Immunogen: Cleaved-APG5-T193 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding pT439 of human APG5.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-APG5 antibody

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Product Categories/Family for anti-APG5 antibody

Antibodies; Abs to Autophagy

NCBI and Uniprot Product Information

• NCBI GI #: 26450228
• NCBI GeneID: 831594
• UniProt Accession #: Q9FFI2
• Molecular Weight: 38,477 Da
• NCBI Official Full Name: APG5 (autophagy 5) like protein
• NCBI Official Symbol: APG5
• NCBI Official Synonym Symbols: ATATG5; ATG5; AUTOPHAGY 5; MKP11.20; MKP11_20
• NCBI Protein Information: autophagy protein 5
• UniProt Protein Name: Autophagy protein 5
• UniProt Gene Name: ATG5
• UniProt Synonym Gene Names: APG5; AtAPG5
• UniProt Entry Name: ATG5_ARATH

Uniprot Description

Function: Required for autophagy. Conjugation to ATG12 is essential for plant nutrient recycling. Ref.6

Subunit structure: Conjugated to ATG12

By similarity.

Subcellular location: Cytoplasm

By similarity.

Tissue specificity: Ubiquitous. Ref.6

Post-translational modification: Conjugated to ATG12; which is essential for autophagy. Conjugation with ATG12 involves ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme

By similarity.

Disruption phenotype: Plants are hypersensitive to nitrogen or carbon starvation and display early senescence. Ref.6

Sequence similarities: Belongs to the ATG5 family.