Rabbit anti-Human, Mouse Bystin Polyclonal Antibody | anti-BYSL antibody

Bystin, CT (BYSL)

Cat. Num. AAA6006479

• Gene Names: BYSL; BYSTIN
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Bystin; Polyclonal Antibody; CT (BYSL); Anti -Bystin; anti-BYSL antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Recognizes human Bystin. Species Crossreactivity
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

• Applicable Applications for anti-BYSL antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1ug/ml; Immunohistochemistry: 1:300
• Immunogen: Synthetic linear peptide corresponding to C-terminal of human Bytsin (KLH).
• Positive Control: HeLa cell lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-BYSL antibody

Bystin and tastin serve as cytoplasmic proteins that are required for trophinin to mediate cell-to-cell adhesion between trophoblastic and endometrial epithelial cells in human placenta. Although the trophinin gene is unique to mammals, the bystin gene (BYSL) is conserved across eukaryotes. It is shown that bystin influences the transition from silent trophectoderm to an active trophoblast through trophinin-mediated cell adhesion. It is also essential for embryonic stem cell survival and trophectoderm development in mouse. Bystin is demonstrated to play a key role in ribosomal biogenesis by processing 18S RNA in the 40S subunit in human cancer cells and mouse embryos. It is also reported that c-MYC targets BYSL, which corresponds with bystin-mediated, rapid protein synthesis, and subsequent cellular growth.

Product Categories/Family for anti-BYSL antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 51173724
• NCBI GeneID: 705
• NCBI Accession #: NP_004044.3
• NCBI GenBank Nucleotide #: NM_004053.3
• UniProt Accession #: Q13895; Q6P5W4; Q86W44; Q96IP8
• Molecular Weight: 49,601 Da
• NCBI Official Full Name: bystin
• NCBI Official Synonym Full Names: bystin-like
• NCBI Official Symbol: BYSL
• NCBI Official Synonym Symbols: BYSTIN
• NCBI Protein Information: bystin; by the ribosomal protein s6 gene, drosophila, homolog-like
• UniProt Protein Name: Bystin
• Protein Family: Bystin
• UniProt Gene Name: BYSL
• UniProt Entry Name: BYST_HUMAN

NCBI Description

Bystin is expressed as a 2-kb major transcript and a 3.6-kb minor transcript in SNG-M cells and in human trophoblastic teratocarcinoma HT-H cells. Protein binding assays determined that bystin binds directly to trophinin and tastin, and that binding is enhanced when cytokeratins 8 and 18 are present. Immunocytochemistry of HT-H cells showed that bystin colocalizes with trophinin, tastin, and the cytokeratins, suggesting that these molecules form a complex in trophectoderm cells at the time of implantation. Using immunohistochemistry it was determined that trophinin and bystin are found in the placenta from the sixth week of pregnancy. Both proteins were localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week 10, the levels of trophinin, tastin, and bystin decreased and then disappeared from placental villi. [provided by RefSeq, Jul 2008]

Uniprot Description

BYSL: Required for processing of 20S pre-rRNA precursor and biogenesis of 40S ribosomal subunits. May be required for trophinin-dependent regulation of cell adhesion during implantation of human embryos. Belongs to the bystin family.

Protein type: Nucleolus; Cell adhesion

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: intracellular membrane-bound organelle; membrane; apical part of cell; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding

Biological Process: maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); cell proliferation; female pregnancy; cell adhesion; trophectodermal cell differentiation