Rabbit anti-Human NUP210 Polyclonal Antibody | anti-NUP210 antibody

NUP210, NT (NUP210, KIAA0906, Nuclear pore membrane glycoprotein 210, Nuclear envelope pore membrane protein POM 210, Nucleoporin Nup210, Pore membrane protein of 210kD) (Azide free) (HRP)

Cat. Num. AAA6327264

• Gene Names: NUP210; GP210; POM210
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: NUP210; Polyclonal Antibody; NT (NUP210; KIAA0906; Nuclear pore membrane glycoprotein 210; Nuclear envelope pore membrane protein POM 210; Nucleoporin Nup210; Pore membrane protein of 210kD) (Azide free) (HRP); Pore membrane protein of 210kD; anti-NUP210 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NUP210.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-NUP210 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 202-231 from the N-terminal region of human NUP210. Species sequence homology: mouse and rat.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of T47D cell line lysates (35ug/lane) usingMBS645881. Arrow indicates NUP210 was detected usingMBS645881.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of formalin fixed and paraffin embedded human lung carcinoma using MBS645881 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MBS645881 for immunohistochemistry.)

Testing Data

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Product Categories/Family for anti-NUP210 antibody

Antibodies; KIAA; orf

References

1. Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010). 2. Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010). 3. Olsen, J.V., et al. Cell 127(3):635-648(2006). 4. Stavru, F., et al. J. Cell Biol. 173(4):477-483(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 27477134
• NCBI GeneID: 23225
• NCBI Accession #: NP_079199.2
• NCBI GenBank Nucleotide #: NM_024923.3
• UniProt Accession #: Q8TEM1; O94980; Q6NXG6; Q8NBJ1; Q9H6C8; Q9UFP3; A6NN56
• Molecular Weight: 105,810 Da
• NCBI Official Full Name: nuclear pore membrane glycoprotein 210
• NCBI Official Synonym Full Names: nucleoporin 210kDa
• NCBI Official Symbol: NUP210
• NCBI Official Synonym Symbols: GP210; POM210
• NCBI Protein Information: nuclear pore membrane glycoprotein 210; nuclear envelope pore membrane protein POM 210; nuclear pore protein gp210; nucleoporin Nup210; pore membrane protein of 210 kDa
• UniProt Protein Name: Nuclear pore membrane glycoprotein 210
• Protein Family: Nuclear pore membrane glycoprotein
• UniProt Gene Name: NUP210
• UniProt Synonym Gene Names: KIAA0906; Nuclear pore protein gp210; POM210
• UniProt Entry Name: PO210_HUMAN

NCBI Description

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a membrane-spanning glycoprotein that is a major component of the nuclear pore complex. Multiple pseudogenes related to this gene are located on chromosome 3. [provided by RefSeq, Jul 2013]

Uniprot Description

NUP210: Nucleoporin essential for nuclear pore assembly and fusion, nuclear pore spacing, as well as structural integrity. Belongs to the NUP210 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleoporin; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p25.1

Cellular Component: endoplasmic reticulum membrane; nuclear membrane; membrane; nuclear envelope; nuclear pore

Molecular Function: protein dimerization activity

Biological Process: mRNA transport; viral reproduction; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; glucose transport; viral infectious cycle; protein transport; hexose transport; carbohydrate metabolic process; viral transcription; gene expression; mitotic cell cycle; transmembrane transport