Western Blot (WB)
(Western Blot analysis of in vitro translated p35 baculovirus protein using MBS6006015 at 1:2000. Full-length p35 is detected at ~35kD, lower molecular p35 breakdown/cleavage bands are also detected.)
Rabbit p35, Baculovirus Polyclonal Antibody
p35, Baculovirus
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Grade: SerumPurity: Serum
Synonyms
p35; Baculovirus; Polyclonal Antibody; Anti -p35; anti-p35; Baculovirus antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes p35 baculovirus.
Purity/Purification
Grade: Serum
Purity: Serum
Purity: Serum
Form/Format
Supplied as a liquid, 0.05% sodium azide.
Applicable Applications for anti-p35, Baculovirus antibody
Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications not tested.
Application Notes
Western Blot: 1:1000-1:2000. p35 baculovirus may be observed as one or multiple bands. p35 baculovirus is cleaved during reaction woith caspases. 10 and 25kD fragments have been described (3,4).
Immunohistochemistry (FFPE): 1:1000-1:5000
Immunohistochemistry: Frozen sections
Immunoprecipitation: 1:50-1:200
Optimal dilutions to be determined by the researcher.
Immunohistochemistry (FFPE): 1:1000-1:5000
Immunohistochemistry: Frozen sections
Immunoprecipitation: 1:50-1:200
Optimal dilutions to be determined by the researcher.
Immunogen
Recombinant corresponding to full-length p35 baculovirus protein.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of in vitro translated p35 baculovirus protein using MBS6006015 at 1:2000. Full-length p35 is detected at ~35kD, lower molecular p35 breakdown/cleavage bands are also detected.)
Western Blot (WB)
(Western Blot analysis of in vitro translated p35 baculovirus protein using MBS6006015 at 1:2000. Full-length p35 is detected at ~35kD, lower molecular p35 breakdown/cleavage bands are also detected.)
Related Product Information for anti-p35, Baculovirus antibody
The baculoviruses are a large, diverse family of DNA viruses that have evolved a number of mechanisms to manipulate thier insect hosts. One of these is the ability to regulate apoptosis during infection by expressing proteins that can inhibit caspase activation, including the caspase inhibitor p35 and the inhibitor of apoptosis (IAP) proteins. The p35 baculovirus protein strongly inhibits caspase enzymatic activity, and is the the most broadly acting caspase inhibitor protein known. p35 baculovirus forms essentially irreversible complexes with its target caspases in a process that is accompanied by the cleavage of p35, generating two fragments of approximately 10kD and 25kD. These cleavage fragments remain associated with caspases and thereby block caspase activity. The ability of p35 to inhibit caspases along with the central role of caspases in the apoptotic process enables p35 baculovirus to block apoptosis in a phylogentically broad range of cells, and in response to a wide variety of apoptotic induction signals. For example, over expression of p35 in mammalian, insect, and nematode cells results in resistance to apoptosis.
Product Categories/Family for anti-p35, Baculovirus antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
P35
Similar Products
Product Notes
The p35, Baculovirus (Catalog #AAA6006015) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's p35, Baculovirus can be used in a range of immunoassay formats including, but not limited to, Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications not tested. Western Blot: 1:1000-1:2000. p35 baculovirus may be observed as one or multiple bands. p35 baculovirus is cleaved during reaction woith caspases. 10 and 25kD fragments have been described (3,4). Immunohistochemistry (FFPE): 1:1000-1:5000 Immunohistochemistry: Frozen sections Immunoprecipitation: 1:50-1:200 Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the p35, Baculovirus for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p35, Baculovirus, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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