Sheep Dopamine Beta Hydroxylase Polyclonal Antibody

Dopamine Beta Hydroxylase, CT (DBH, Dopamine beta Monooxygenase)

Cat. Num. AAA6005596

• Reactivity: Bovine, Human, Monkey, Rabbit
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Dopamine Beta Hydroxylase; Polyclonal Antibody; CT (DBH; Dopamine beta Monooxygenase); Anti -Dopamine Beta Hydroxylase; anti-Dopamine Beta Hydroxylase antibody

• Host: Sheep
• Reactivity: Bovine, Human, Monkey, Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Dopamine Beta Hydroxylase. Reacts specifically with a single (or double) band(s) of Mr ~70-75,000 in samples of SDS-solubilized, DTT-reduced human adrenal medulla subjected to SDS-PAGE. Species Crossreactivity: monkey, bovine, rabbit (slight).
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.09% sodium azide, 50% glycerol.

• Applicable Applications for anti-Dopamine Beta Hydroxylase antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:250-1:1000 on human, monkey and bovine adrenal medulla.; Immunohistochemistry: 1:250-1:1000, staining was evaluated in the locus coeruleus of formaldehyde-fixed, cryopreserved human and monkey brain. Showed strong staining on both human and monkey.
• Immunogen: Synthetic peptide corresponding to CPTSQGRSPAGPTVVSI-amide, from C-terminal region of human Dopamine Beta Hydroxylase.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-Dopamine Beta Hydroxylase antibody

The production of neurotransmitters is controlled by environmental stimuli, which send signals through second messengers and protein kinase pathways. Regulation of gene expression of dopamine beta hydroxylase and tyrosine hydroxylase, two of the catecholamine biosynthetic enzymes, by cyclic AMP, diacyl glycerol, and Ca2+ leads to increased neurotransmitter biosynthesis. Dopamine beta-hydroxylase catalyzes the conversion of dopamine to noradrenaline and is expressed selectively in noradrenergic and adrenergic neurons in the nervous system. The disruption of the DBH gene is thought to cause changes in development and behavior in certain models. Both DBH and the multiple regulatory pathways of DBH are of increasing research in many neurological diseases.

Product Categories/Family for anti-Dopamine Beta Hydroxylase antibody

Antibodies; Abs to Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 2358082
• NCBI Official Full Name: dopamine-beta-hydroxylase

Uniprot Description

MOXD2: Belongs to the copper type II ascorbate-dependent monooxygenase family.

Protein type: Membrane protein, integral; Oxidoreductase; EC 1.14.17.-

Cellular Component: membrane; integral to membrane

Molecular Function: copper ion binding; metal ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced ascorbate as one donor, and incorporation of one atom of oxygen; oxidoreductase activity; monooxygenase activity; catalytic activity