Mouse anti-Human, Mouse EphA4 Receptor Monoclonal Antibody | anti-Epha4 antibody

EphA4 Receptor (Ephrin A4 Receptor)

Cat. Num. AAA600505

• Gene Names: Epha4; rb; Sek; Cek8; Hek8; Sek1; Tyro1; AI385584; 2900005C20Rik
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: EphA4 Receptor; Monoclonal Antibody; EphA4 Receptor (Ephrin A4 Receptor); Anti -EphA4 Receptor (Ephrin A4 Receptor); anti-Epha4 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 6A70
• Specificity: Recognizes the endogenous human EphA4 receptor protein. No crossreactivity with EphA5 or EphA7 has been detected. On Western Blots, this antibody identifies a band at ~110-120kD. Reactivity has been confirmed with EphA4-transfected 293T cells and mouse brain lysates by Western Blot and Immunoprecipitation and on transfected 293 cells and mouse hippocampus by immunohistochemistry.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-Epha4 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.; Dilution: Western Blot (Human, mouse, chicken): 1-3ug/ml; Immunoprecipitation (Human, mouse, chicken): 5ug; Immunohistochemistry (Frozen, mouse): 3-10ug/ml
• Immunogen: Synthetic peptide corresponding to the C-terminus of human EphA4 receptor protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-Epha4 antibody

The Eph receptors are the largest family of receptor tyrosine kinases. They can be grouped into two subclasses based on sequence homology: the EphA and the EphB receptors. The ligands for the Eph receptors are the ephrins. The EphA receptors bind ephrin-A ligands, which are tethered to the membrane by a GPI linkage, and the EphB receptors bind ephrin-B ligands, which have a membrane spanning region. The Eph receptors are widely expressed during embryonic

Product Categories/Family for anti-Epha4 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 34328113
• NCBI GeneID: 13838
• NCBI Accession #: NP_031962.2
• NCBI GenBank Nucleotide #: NM_007936.3
• UniProt Accession #: Q03137; Q80VZ2
• Molecular Weight: 109,814 Da
• NCBI Official Full Name: ephrin type-A receptor 4
• NCBI Official Synonym Full Names: Eph receptor A4
• NCBI Official Symbol: Epha4
• NCBI Official Synonym Symbols: rb; Sek; Cek8; Hek8; Sek1; Tyro1; AI385584; 2900005C20Rik
• NCBI Protein Information: ephrin type-A receptor 4; tyrosine-protein kinase receptor MPK-3; tyrosine-protein kinase receptor SEK-1
• UniProt Protein Name: Ephrin type-A receptor 4
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: Epha4
• UniProt Synonym Gene Names: Sek; Sek1
• UniProt Entry Name: EPHA4_MOUSE

Uniprot Description

EphA4: a tyrosine kinase receptor of the Eph family. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A4 and -A5. Binds more poorly to ephrin-A2 and -A3. May play a role in hindbrain pattern formation. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels.

Protein type: Protein kinase, TK; Kinase, protein; Membrane protein, integral; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; Eph family

Cellular Component: Golgi apparatus; cell surface; endoplasmic reticulum; integral to plasma membrane; postsynaptic density; dendrite; dendritic spine; integral to membrane; perikaryon; postsynaptic membrane; mitochondrial outer membrane; cell projection; membrane; early endosome membrane; axon; cytoplasm; plasma membrane; synapse; nerve terminal; neuromuscular junction; cell junction; endosome; filopodium

Molecular Function: identical protein binding; transmembrane-ephrin receptor activity; ephrin receptor binding; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; protein kinase activity; transferase activity; protein binding; protein-tyrosine kinase activity; GPI-linked ephrin receptor activity; transferase activity, transferring phosphorus-containing groups; ephrin receptor activity; kinase activity; PH domain binding; ATP binding

Biological Process: positive regulation of JNK activity; nervous system development; axon guidance; regulation of GTPase activity; peptidyl-tyrosine phosphorylation; negative regulation of axon regeneration; multicellular organismal development; protein amino acid autophosphorylation; motor axon guidance; regulation of astrocyte differentiation; positive regulation of dendrite morphogenesis; glial cell migration; regulation of axonogenesis; adult walking behavior; protein amino acid phosphorylation; ephrin receptor signaling pathway; corticospinal tract morphogenesis; cell adhesion; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway