Goat anti-Human GABRB3 Polyclonal Antibody | anti-GABRB3 antibody

GABRB3, ID (Gamma-aminobutyric Acid Receptor Subunit beta-3, GABA(A) Receptor Subunit beta-3)

Cat. Num. AAA6004846

• Gene Names: GABRB3; ECA5
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: GABRB3; Polyclonal Antibody; ID (Gamma-aminobutyric Acid Receptor Subunit beta-3; GABA(A) Receptor Subunit beta-3); Anti -GABRB3; anti-GABRB3 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human Gabrb3. Species sequence homology: Bovine, canine, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-GABRB3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, ELISA and Immunohistochemistry.; Dilution: ELISA: 1:8000; Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml
• Immunogen: Synthetic peptide corresponding to C-EKTAKAKNDRSK from the internal region of human Gabrb3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with GABRB3 antibody followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-GABRB3 antibody

GABRB3 is a member of the ligand-gated ionic channel family. This protein is one of at least 13 distinct subunits of a multisubunit chloride channel that serves as the receptor for gamma-aminobutyric acid, the major inhibitory transmitter of the nervous system. Mutations in this gene may be associated with the pathogenesis of Angelman syndrome, Prader-Willi syndrome, and autism.

Product Categories/Family for anti-GABRB3 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 522838289
• NCBI GeneID: 2562
• NCBI Accession #: NP_001265560.1
• NCBI GenBank Nucleotide #: NM_001278631.1
• UniProt Accession #: P28472; Q14352; Q96FM5; B7Z2W1; B7Z825; F5H3D2; H7BYV8
• Molecular Weight: 54,116 Da
• NCBI Official Full Name: gamma-aminobutyric acid receptor subunit beta-3 isoform 3
• NCBI Official Synonym Full Names: gamma-aminobutyric acid (GABA) A receptor, beta 3
• NCBI Official Symbol: GABRB3
• NCBI Official Synonym Symbols: ECA5
• NCBI Protein Information: gamma-aminobutyric acid receptor subunit beta-3; GABAA receptor beta-3 subunit; GABA-alpha receptor beta-2 subunit
• UniProt Protein Name: Gamma-aminobutyric acid receptor subunit beta-3
• Protein Family: Gamma-aminobutyric acid receptor
• UniProt Gene Name: GABRB3
• UniProt Entry Name: GBRB3_HUMAN

NCBI Description

This gene encodes a member of the ligand-gated ionic channel family. The encoded protein is one the subunits of a multi-subunit chloride channel that serves as the receptor for gamma-aminobutyric acid, a major inhibitory neurotransmitter of the mammalian nervous system. This gene is located on the long arm of chromosome 15 in a cluster with two other genes encoding related subunits of the family. This gene may be associated with the pathogenesis of several disorders including Angelman syndrome, Prader-Willi syndrome, nonsyndromic orofacial clefts, epilepsy and autism. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2013]

Uniprot Description

GABRB3: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Defects in GABRB3 are associated with chronic insomnia, a condition of inability to initiate or maintain sleep. This may occur as a primary disorder or in association with another medical or psychiatric condition. Defects in GABRB3 are the cause of childhood absence epilepsy type 5 (ECA5). ECA5 is a subtype of idiopathic generalized epilepsy (IGE) characterized by an onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures develop. Absence seizures may either remit or persist into adulthood. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB3 sub-subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Membrane protein, integral; Channel, chloride; Membrane protein, multi-pass; Channel, ligand-gated; Transporter, ion channel

Chromosomal Location of Human Ortholog: 15q12

Cellular Component: postsynaptic membrane; integral to plasma membrane; plasma membrane; cell junction

Molecular Function: chloride channel activity; GABA-A receptor activity; GABA-gated chloride ion channel activity; extracellular ligand-gated ion channel activity

Biological Process: synaptic transmission; sensory perception of sound; inner ear receptor cell development; transport; negative regulation of neuron apoptosis; palate development; signal transduction; transmembrane transport

Disease: Epilepsy, Childhood Absence, Susceptibility To, 5