Mouse anti-Human Complement C3, Activated Monoclonal Antibody | anti-C5 antibody

Complement C3, Activated

Cat. Num. AAA6004133

• Gene Names: C5; C5a; C5b; CPAMD4
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Complement C3; Activated; Monoclonal Antibody; Anti -Complement C3; anti-C5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: bH6
• Specificity: Recognizes a neo-epitope expressed on the cleavage fragments of C3b, iC3b and C3c, but not C3dg and C3f.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as as liquid in PBS, 0.1% BSA, 0.02% sodium azide.

• Applicable Applications for anti-C5 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA: Detects and/or quantifies C3 activation products in human plasma, synovial fluid and cerebrospinal fluid; Immunohistochemistry (Paraffin, frozen): 1:50
• Immunogen: zymosan-activated serum
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-C5 antibody

The complement system is an important factor in innate immunity. The third complement component, C3, is central to the classical, alternative and lectin pathways of complement activation. Activation products of the complement cascade contain neo-epitopes that are not present in the individual native components. Monoclonal antibodies detecting neo-epitopes have been used for direct quantification of activation at different steps in the complement cascade. The synthesis of C3 is tissue-specific and is modulated in response to a variety of stimulatory agents. C3 is the most abundant protein of the complement system with serum protein levels of about 1.3mg/ml. An inherited deficiency of C3 predisposes the person to frequent assaults of bacterial infections. In ulcerative colitis and idiopathic chronic inflammatory bowel disease, the deposition of C3 in the diseased mucosa has been reported.

Product Categories/Family for anti-C5 antibody

Antibodies; Abs to Complement

NCBI and Uniprot Product Information

• NCBI GI #: 38016947
• NCBI GeneID: 727
• NCBI Accession #: NP_001726.2
• NCBI GenBank Nucleotide #: NM_001735.2
• UniProt Accession #: P01031; Q14CJ0; Q27I61
• Molecular Weight: 188,305 Da
• NCBI Official Full Name: complement C5 preproprotein
• NCBI Official Synonym Full Names: complement component 5
• NCBI Official Symbol: C5
• NCBI Official Synonym Symbols: C5a; C5b; CPAMD4
• NCBI Protein Information: complement C5; prepro-C5; C5a anaphylatoxin; anaphylatoxin C5a analog; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
• UniProt Protein Name: Complement C5
• Protein Family: Complement C5
• UniProt Gene Name: C5
• UniProt Synonym Gene Names: CPAMD4
• UniProt Entry Name: CO5_HUMAN

NCBI Description

The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to a susceptibility to liver fibrosis and to rheumatoid arthritis. [provided by RefSeq, Jul 2008]

Uniprot Description

C5: Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Defects in C5 are the cause of complement component 5 deficiency (C5D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q33-q34

Cellular Component: membrane attack complex; extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity; endopeptidase inhibitor activity; receptor binding; C5a anaphylatoxin chemotactic receptor binding

Biological Process: in utero embryonic development; positive regulation of chemotaxis; activation of MAPK activity; cytolysis; complement activation, alternative pathway; glucose homeostasis; chemotaxis; leukocyte migration during inflammatory response; complement activation; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; cell surface receptor linked signal transduction; regulation of complement activation; innate immune response; response to stress; inflammatory response; negative regulation of dopamine secretion; complement activation, classical pathway

Disease: Complement Component 5 Deficiency; Eculizumab, Poor Response To