Rabbit anti-Human, Mouse WIBG Polyclonal Antibody | anti-WIBG antibody

WIBG, NT (WIBG, PYM, Partner of Y14 and mago, Protein wibg homolog)

Cat. Num. AAA6002996

• Gene Names: WIBG; PYM
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: WIBG; Polyclonal Antibody; NT (WIBG; PYM; Partner of Y14 and mago; Protein wibg homolog); Anti -WIBG; anti-WIBG antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-WIBG antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100
• Immunogen: WIBG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-44 amino acids from the N-terminal region of human WIBG.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(WIBG Antibody (N-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the WIBG antibody detected the WIBG protein (arrow).)

Immunohistochemistry (IHC)

(WIBG antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervical tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WIBG antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-WIBG antibody

Key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs and serves as a positional landmarks for the intron exon structure of genes and directs post-transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) or translation. Acts as a EJC disassembly factor, allowing translation-dependent EJC removal and recycling by disrupting mature EJC from spliced mRNAs. Its association with the 40S ribosomal subunit probably prevents a translation-independent disassembly of the EJC from spliced mRNAs, by restricting its activity to mRNAs that have been translated. Interferes with NMD and enhances translation of spliced mRNAs, probably by antagonizing EJC functions. May bind RNA; the relevance of RNA-binding remains unclear in vivo, RNA-binding was detected by PubMed:14968132, while PubMed:19410547 did not detect RNA-binding activity independently of the EJC.

Product Categories/Family for anti-WIBG antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 16307094
• NCBI GeneID: 84305
• UniProt Accession #: Q9BRP8; Q8IXJ8; Q8N8E7; B6ZDM5
• Molecular Weight: 22,656 Da
• NCBI Official Full Name: WIBG protein, partial
• NCBI Official Synonym Full Names: within bgcn homolog (Drosophila)
• NCBI Official Symbol: WIBG
• NCBI Official Synonym Symbols: PYM
• NCBI Protein Information: partner of Y14 and mago; protein wibg homolog
• UniProt Protein Name: Partner of Y14 and mago
• UniProt Gene Name: WIBG
• UniProt Synonym Gene Names: PYM
• UniProt Entry Name: WIBG_HUMAN

Uniprot Description

WIBG: a conserved protein of unknown function. A potential RNA-binding protein.

Protein type: RNA-binding; Nucleolus; Translation

Chromosomal Location of Human Ortholog: 12q13.2

Cellular Component: nucleoplasm; cytoplasm; nucleolus

Molecular Function: protein binding; ribosome binding

Biological Process: positive regulation of translation; mRNA catabolic process, nonsense-mediated decay