Western Blot (WB)
(Western blot analysis of lysates from U-251 MG, HL-60 cell line (from left to right), using WIBG Antibody (N-term). MBS9210473 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Rabbit WIBG Polyclonal Antibody | anti-WIBG antibody
WIBG Antibody (N-term)
Gene Names
WIBG; PYM
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
WIBG; Polyclonal Antibody; WIBG Antibody (N-term); Partner of Y14 and mago; Protein wibg homolog; PYM; anti-WIBG antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This WIBG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-40 amino acids from the N-terminal region of human WIBG.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
12-40
Sequence Length
203
Applicable Applications for anti-WIBG antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of lysates from U-251 MG, HL-60 cell line (from left to right), using WIBG Antibody (N-term). MBS9210473 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from U-251 MG, HL-60 cell line (from left to right), using WIBG Antibody (N-term). MBS9210473 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Immunohistochemistry (IHC)
(WIBG antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervical tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WIBG antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(WIBG antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervical tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WIBG antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-WIBG antibody
Key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs and serves as a positional landmarks for the intron exon structure of genes and directs post-transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) or translation. Acts as a EJC disassembly factor, allowing translation-dependent EJC removal and recycling by disrupting mature EJC from spliced mRNAs. Its association with the 40S ribosomal subunit probably prevents a translation-independent disassembly of the EJC from spliced mRNAs, by restricting its activity to mRNAs that have been translated. Interferes with NMD and enhances translation of spliced mRNAs, probably by antagonizing EJC functions. May bind RNA; the relevance of RNA-binding remains unclear in vivo, RNA-binding was detected by PubMed:14968132, while PubMed:19410547 did not detect RNA-binding activity independently of the EJC.
References
Gehring, N.H., et al. Cell 137(3):536-548(2009)
Diem, M.D., et al. Nat. Struct. Mol. Biol. 14(12):1173-1179(2007)
Forler, D., et al. Nat. Biotechnol. 21(1):89-92(2003)
Gatfield, D., et al. J. Cell Biol. 159(4):579-588(2002)
Diem, M.D., et al. Nat. Struct. Mol. Biol. 14(12):1173-1179(2007)
Forler, D., et al. Nat. Biotechnol. 21(1):89-92(2003)
Gatfield, D., et al. J. Cell Biol. 159(4):579-588(2002)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22656
NCBI Official Full Name
partner of Y14 and mago isoform 2
NCBI Official Synonym Full Names
within bgcn homolog (Drosophila)
NCBI Official Symbol
WIBG
NCBI Official Synonym Symbols
PYM
NCBI Protein Information
partner of Y14 and mago
UniProt Protein Name
Partner of Y14 and mago
UniProt Gene Name
WIBG
UniProt Synonym Gene Names
PYM
UniProt Entry Name
WIBG_HUMAN
Uniprot Description
WIBG: a conserved protein of unknown function. A potential RNA-binding protein.
Protein type: RNA-binding; Translation; Nucleolus
Chromosomal Location of Human Ortholog: 12q13.2
Cellular Component: nucleoplasm; cytoplasm; nucleolus
Molecular Function: protein binding; ribosome binding
Biological Process: positive regulation of translation; mRNA catabolic process, nonsense-mediated decay
Research Articles on WIBG
Similar Products
Product Notes
The WIBG wibg (Catalog #AAA9210473) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 12-40. The WIBG Antibody (N-term) reacts with Human (Predicted Reactivity: Bovine, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's WIBG can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the WIBG wibg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WIBG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
