Immunohistochemistry (IHC)
(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit anti-Human HES1 Polyclonal Antibody | anti-C21orf33 antibody
HES1, NT (Hairy and Enhancer of Split 1, HES-1, bHLHb39, Class B Basic Helix-loop-helix Protein 39, FLJ20408, Hairy Homolog, Hairy-like Protein, hHL, HL, HRY, Transcription Factor HES-1)
Gene Names
C21orf33; ES1; HES1; KNPH; KNPI; GT335
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
HES1; Polyclonal Antibody; NT (Hairy and Enhancer of Split 1; HES-1; bHLHb39; Class B Basic Helix-loop-helix Protein 39; FLJ20408; Hairy Homolog; Hairy-like Protein; hHL; HL; HRY; Transcription Factor HES-1); Anti -HES1; anti-C21orf33 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human HES1. Species sequence homology: mouse and rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Applicable Applications for anti-C21orf33 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: ELISA: 1:80,000
Western Blot: 1:200-1:1000
Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml
Dilution: ELISA: 1:80,000
Western Blot: 1:200-1:1000
Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml
Immunogen
Synthetic peptide corresponding to human HES1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))
Western Blot (WB)
(Western Blot analysis of Raji cell lysates with HES-1 antibody.)
Western Blot (WB)
(Western Blot analysis of Raji cell lysates with HES-1 antibody.)
Related Product Information for anti-C21orf33 antibody
HES1 is a nuclear protein belonging to th basic helix-loop-helix family of transcription factors with a basic helix-loop-helix (bHLH) domain and an Orange domain. The basic domain of HES1 contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. An immediate early response gene to growth factor, HES1 is reported as having a dual function of both transcriptional activator and silencer of acid alpha-glucosidase (GAA), thus contributing to the subtle tissue-specific control of housekeeping genes such as GAA. HES1 is a transcriptional repressor of genes which require a bHLH protein for their transcription and also the formation of a complex with a corepressor protein (Groucho) such as TLE2. It may act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1.
Product Categories/Family for anti-C21orf33 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
28,170 Da
NCBI Official Full Name
HES1
NCBI Official Synonym Full Names
chromosome 21 open reading frame 33
NCBI Official Symbol
C21orf33
NCBI Official Synonym Symbols
ES1; HES1; KNPH; KNPI; GT335
NCBI Protein Information
ES1 protein homolog, mitochondrial; Keio novel protein I; human HES1 protein, homolog to E.coli and zebrafish ES1 protein
UniProt Protein Name
ES1 protein homolog, mitochondrial
UniProt Gene Name
C21orf33
UniProt Synonym Gene Names
HES1; KNPI
UniProt Entry Name
ES1_HUMAN
NCBI Description
This gene encodes a potential mitochondrial protein that is a member of the DJ-1/PfpI gene family. This protein is overexpressed in fetal Down syndrome brain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
Uniprot Description
C21orf33: a mitochondrial protein. Two alternatively spliced isoforms have been described.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: mitochondrion
Research Articles on C21orf33
Similar Products
Product Notes
The C21orf33 c21orf33 (Catalog #AAA6002042) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HES1, NT (Hairy and Enhancer of Split 1, HES-1, bHLHb39, Class B Basic Helix-loop-helix Protein 39, FLJ20408, Hairy Homolog, Hairy-like Protein, hHL, HL, HRY, Transcription Factor HES-1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HES1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot and Immunohistochemistry. Dilution: ELISA: 1:80,000 Western Blot: 1:200-1:1000 Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml. Researchers should empirically determine the suitability of the C21orf33 c21orf33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HES1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
