Western Blot (WB)
(SP6 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SP6 antibody detected the SP6 protein (arrow).)
Rabbit anti-Human SP6 Polyclonal Antibody | anti-sp6 antibody
SP6, ID (SP6, KLF14, Transcription factor Sp6, Krueppel-like factor 14)
Gene Names
sp6; xsp6
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
SP6; Polyclonal Antibody; ID (SP6; KLF14; Transcription factor Sp6; Krueppel-like factor 14); Anti -SP6; anti-sp6 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-sp6 antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Immunogen
SP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-225 amino acids from the Central region of human SP6.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(SP6 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SP6 antibody detected the SP6 protein (arrow).)
Western Blot (WB)
(SP6 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SP6 antibody detected the SP6 protein (arrow).)
Flow Cytometry (FC/FACS)
(SP6 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(SP6 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-sp6 antibody
SP6 belongs to a family of transcription factors that contain 3 classical zinc finger DNA-binding domains consisting of a zinc atom tetrahedrally coordinated by 2 cysteines and 2 histidines (C2H2 motif). These transcription factors bind to GC-rich sequences and related GT and CACCC boxes (Scohy et al., 2000 [PubMed 11087666]).
Product Categories/Family for anti-sp6 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Sp6 transcription factor
NCBI Official Synonym Full Names
Sp6 transcription factor<
NCBI Official Symbol
sp6
NCBI Official Synonym Symbols
xsp6
NCBI Protein Information
Sp6 transcription factor
Protein Family
Similar Products
Product Notes
The sp6 (Catalog #AAA6001794) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SP6, ID (SP6, KLF14, Transcription factor Sp6, Krueppel-like factor 14) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SP6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the sp6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SP6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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