Western Blot (WB)
(Western Blot analysis of extracts from various cell lines using MBS6001760.)
Rabbit Histone H2B, ubiquitylated Monoclonal Antibody | anti-hist1h2bj antibody
Histone H2B, ubiquitylated (Lys120) (Histone H2B Type 1-B, Histone H2B.f, H2B/f, H2BFF, Histone H2B.1, H2B.1, Histone Cluster 1 H2bb, HIST1H2BB, MGC119804)
Purified
Purified
Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Chromatin IP: 1:50
Western Blot (WB)
(Western Blot analysis of extracts from various cell lines using MBS6001760.)
Western Blot (WB)
(Western Blot analysis of extracts from various cell lines using MBS6001760.)
Immunoprecipitation (IP)
(Chromatin immunoprecipitations were performed with cross-linked chromatin from 4x10e6 HeLa cells and either 5ul of MBS6001760 Histone H2B, ubiquitylated (Lys120) or 2ul of Normal Rabbit IgG using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads). The enriched DNA was quantified by real-time PCR using SimpleChIP® Human γ-Actin Promoter Primers, SimpleChIP® Human γ-Actin Intron 3 Primers, SimpleChIP® Human GAPDH Promoter Primers, and SimpleChIP® Human GAPDH Intron 2 Primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.)
Immunoprecipitation (IP)
(Chromatin immunoprecipitations were performed with cross-linked chromatin from 4x10e6 HeLa cells and either 5ul of MBS6001760 Histone H2B, ubiquitylated (Lys120) or 2ul of Normal Rabbit IgG using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads). The enriched DNA was quantified by real-time PCR using SimpleChIP® Human γ-Actin Promoter Primers, SimpleChIP® Human γ-Actin Intron 3 Primers, SimpleChIP® Human GAPDH Promoter Primers, and SimpleChIP® Human GAPDH Intron 2 Primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of HeLa cells using MBS6001760 (green) and COX IV Mouse mAb (red).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of HeLa cells using MBS6001760 (green) and COX IV Mouse mAb (red).)
NCBI and Uniprot Product Information
Uniprot Description
Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Subunit structure: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Ref.5 Ref.6 Ref.7
Subcellular location: Nucleus. Chromosome.
Post-translational modification: Monoubiquitination of Lys-121 by BRE1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation
By similarity.Phosphorylated on Ser-15 during developmentally programmed apoptosis; which may facilitate apoptotic chromatin condensation. Ref.4GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes
By similarity.
Sequence similarities: Belongs to the histone H2B family.
Research Articles on hist1h2bj
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Product Notes
The hist1h2bj h2b1.1 (Catalog #AAA6001760) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2B, ubiquitylated (Lys120) (Histone H2B Type 1-B, Histone H2B.f, H2B/f, H2BFF, Histone H2B.1, H2B.1, Histone Cluster 1 H2bb, HIST1H2BB, MGC119804) reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2B, ubiquitylated can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot and Chromatin IP. Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Chromatin IP: 1:50. Researchers should empirically determine the suitability of the hist1h2bj h2b1.1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2B, ubiquitylated, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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