Norovirus GI capsid C-terminal Recombinant Protein
Human Norovirus GI capsid C-terminal (P domain)
Applications
ELISA, Western Blot
Purity
>95% pure (10% SDS-PAGE, Coomassie stain). Proprietary chromatographic technique
Synonyms
Norovirus GI capsid C-terminal; Human Norovirus GI capsid C-terminal (P domain); Norovirus GI capsid C-terminal recombinant protein
Host
E Coli
Specificity
>95% pure (10% SDS-PAGE, Coomassie stain). Proprietary chromatographic technique
Purity/Purification
>95% pure (10% SDS-PAGE, Coomassie stain). Proprietary chromatographic technique
Form/Format
Purified, Liquid. PBS with 1 M urea (0.05% NaN3)
Concentration
0.45mg/ml (lot 08-2017) (varies by lot)
Applicable Applications for Norovirus GI capsid C-terminal recombinant protein
ELISA (EIA), Western Blot (WB), Vaccine Study
Species
Human
Important Note
Centrifuge before opening to ensure complete recovery of vial contents.
Preparation and Storage
Upon receipt, store at -20 degree C. Avoid multiple freeze/thaw cycles
Related Product Information for Norovirus GI capsid C-terminal recombinant protein
Human norovirus can be classified into two groups, group 1 and group 2. The species Norwalk virus belonging to group 1 was found at Ohio 1968. Norovirus is a common virus to cause human gastroenteritis with symptoms vomiting, diarrhea and sickness. CDC report showed that there are 19 to 21 million Americans infected by Nororvirus annually with 800 deaths, i.e. 1 in 15 people with infection; world-widely, this virus affects around 267 million people and causes over 200,000 deaths each year; these deaths are usually in less developed countries and in the very young, elderly and immuno-suppressed, however, most cases are self-limited with a full recovery within a few days.
Norovirus is highly contagious and can spread from person to person through infected food or water or contaminated surfaces. Outbreaks typically occur from November to April, peaking in January.
Norovirus is a positive sense RNA virus with 7.5 kb nucleotides, encoding a major structural protein VP1 with 50-55kDa. The full length of VP1 capsid contains three regions, S, hinge and P domain. N-terminal 225 amino acids is an essential element forming icosahedron.
P domain from 225 to 520 forms P1- P2-P1structure. P domain has a receptor binding region to recognize human histo-blood group antigens (HBGAs). P domain expressed in bacteria can spontaneously form a P dime and a P particle aggregated by 12 P dimmers. P particle displays an enhanced binding activity to HBGAs higher than virus-like particle (VLP) formed by the full length capsid.
Most immunoreactive eptiopes are located spanning from 275 to 417 amino acids, the peptide from 275 to 417 amino acids is directly exposed to outside to bind to the specific antibody.
P domain is also a good candidate for norovirus vaccine development. Sequence of group I Norwalk strain capsid from 225 to 520 amino acids is expressed in E Coli with 30KDa, a 6 x his tag linked to its N-terminus.
Norovirus is highly contagious and can spread from person to person through infected food or water or contaminated surfaces. Outbreaks typically occur from November to April, peaking in January.
Norovirus is a positive sense RNA virus with 7.5 kb nucleotides, encoding a major structural protein VP1 with 50-55kDa. The full length of VP1 capsid contains three regions, S, hinge and P domain. N-terminal 225 amino acids is an essential element forming icosahedron.
P domain from 225 to 520 forms P1- P2-P1structure. P domain has a receptor binding region to recognize human histo-blood group antigens (HBGAs). P domain expressed in bacteria can spontaneously form a P dime and a P particle aggregated by 12 P dimmers. P particle displays an enhanced binding activity to HBGAs higher than virus-like particle (VLP) formed by the full length capsid.
Most immunoreactive eptiopes are located spanning from 275 to 417 amino acids, the peptide from 275 to 417 amino acids is directly exposed to outside to bind to the specific antibody.
P domain is also a good candidate for norovirus vaccine development. Sequence of group I Norwalk strain capsid from 225 to 520 amino acids is expressed in E Coli with 30KDa, a 6 x his tag linked to its N-terminus.
Product Categories/Family for Norovirus GI capsid C-terminal recombinant protein
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Product Notes
The Norovirus GI capsid C-terminal (Catalog #AAA596163) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Norovirus GI capsid C-terminal can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Vaccine Study. Researchers should empirically determine the suitability of the Norovirus GI capsid C-terminal for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Norovirus GI capsid C-terminal, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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