Interferon-Gamma ELISA Kit | IFN-gamma elisa kit

Interferon-Gamma (IFN-gamma)

Cat. Num. AAA590059

• Gene Names: IFNG; IFG; IFI
• Synonyms: Interferon-Gamma; Interferon-Gamma (IFN-gamma); Interferon-Gamma (IFN-g); IFN-gamma elisa kit

• Sequence Length: 166

• Samples: Mouse serum and Cell Culture Supernatant
• Assay Type: Sandwich
• Sensitivity: The minimum detectable dose of mouse IFN-gamma was determined by adding two standard deviations to the mean optical density value of the 20 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose using a standard curve generated with Calibrator Diluent I is 13.1 pg/mL and using Calibrator Diluent II is 7.5 pg/mL.
• Intra-assay Precision: To determine within-run precision, three different samples of known concentration were assayed by replicates of 8 in 1 assay.
• Intended Uses: This Mouse IFN-gamma ELISA kit is to be used for the in vitro quantitative determination of mouse interferon-gamma concentrations in mouse serum and cell culture supernatant. This kit is intended FOR LABORATORY RESEARCH USE ONLY and is not for use in therapeutic procedures.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IFN-gamma elisa kit

Principle of the assay: This mouse IFN-gamma enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been precoated with a monoclonal specific for mouse IFN-gamma. Standards or samples are then added to the appropriate microtiter plate wells and incubated. In order to quantitatively determine the amount of mouse IFN-gamma present in the sample, a biotin-conjugated antibody specific for mouse IFN-gamma is added to each well to "sandwich" the IFN-gamma immobilized during the first incubation. The microtiter plate then undergoes a second incubation and the wells are thoroughly washed to remove all unbound Biotin-conjugated antibodies. Avidin can bind to biotin with high affinity and amplify the biotin signal. A preparation of avidin conjugated HRP is added to each well and incubated. The avidin HRP conjugate will bind to wells containing biotin. After washing the wells, the unbounded avidin-HRP conjugate is removed. A TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IFN-gamma and biotin-conjugated antibody, avidin conjugated HRP will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450nm +/- 2nm. In order to measure the concentration of mouse IFN-gamma in the samples this kit contains two calibration diluents. (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant). According to the testing system, the provided standard is 2 x fold serial diluted with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D.) versus mouse IFN-gamma concentration (pg/mL). The concentration of mouse IFN-gamma in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for IFN-gamma elisa kit

ELISA Kits for Mouse Cytokines and Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 56786138
• NCBI GeneID: 3458
• NCBI Accession #: NP_000610.2
• NCBI GenBank Nucleotide #: NM_000619.2
• Molecular Weight: 19,348 Da
• NCBI Official Full Name: interferon gamma
• NCBI Official Synonym Full Names: interferon, gamma
• NCBI Official Symbol: IFNG
• NCBI Official Synonym Symbols: IFG; IFI
• NCBI Protein Information: interferon gamma; IFN-gamma; immune interferon
• UniProt Protein Name: Interferon gamma
• UniProt Gene Name: IFNG
• UniProt Entry Name: IFNG_HUMAN

NCBI Description

This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.[provided by RefSeq, Nov 2009]

Uniprot Description

IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family.

Protein type: Secreted, signal peptide; Membrane protein, integral; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 12q14

Cellular Component: extracellular space; extracellular region; external side of plasma membrane

Molecular Function: interferon-gamma receptor binding; cytokine activity

Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; positive regulation of interleukin-23 production; negative regulation of smooth muscle cell proliferation; positive regulation of osteoclast differentiation; positive regulation of interleukin-12 production; apoptosis; positive regulation of interleukin-6 biosynthetic process; negative regulation of epithelial cell differentiation; positive regulation of killing of cells of another organism; negative regulation of transcription from RNA polymerase II promoter; sensory perception of mechanical stimulus; positive regulation of interleukin-1 beta secretion; positive regulation of membrane protein ectodomain proteolysis; cell surface receptor linked signal transduction; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to drug; positive regulation of synaptic transmission, cholinergic; neutrophil chemotaxis; adaptive immune response; negative regulation of myelination; CD8-positive, alpha-beta T cell differentiation during immune response; unfolded protein response; response to virus; cytokine and chemokine mediated signaling pathway; defense response to protozoan; positive regulation of tumor necrosis factor production; humoral immune response; antigen processing and presentation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of chemokine biosynthetic process; positive regulation of interleukin-12 biosynthetic process; negative regulation of interleukin-17 production; protein import into nucleus, translocation; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; cell motility; regulation of insulin secretion

Disease: Hepatitis C Virus, Susceptibility To; Aplastic Anemia; Mycobacterium Tuberculosis, Susceptibility To; Tuberous Sclerosis 2; Human Immunodeficiency Virus Type 1, Susceptibility To