Rabbit anti-Human GPRC5B Polyclonal Antibody | anti-GPRC5B antibody

GPRC5B, CT (GPRC5B, RAIG2, G-protein coupled receptor family C group 5 member B, A-69G12.1, Retinoic acid-induced gene 2 protein) (Azide free) (HRP)

Cat. Num. AAA6304122

• Gene Names: GPRC5B; RAIG2; RAIG-2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GPRC5B; Polyclonal Antibody; CT (GPRC5B; RAIG2; G-protein coupled receptor family C group 5 member B; A-69G12.1; Retinoic acid-induced gene 2 protein) (Azide free) (HRP); Retinoic acid-induced gene 2 protein; anti-GPRC5B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-GPRC5B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GPRC5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 292-321 amino acids from the C-terminal region of human GPRC5B.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GPRC5B Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the GPRC5B antibody detected the GPRC5B protein (arrow).)

Related Product Information for anti-GPRC5B antibody

GPRC5B is a member of the type 3 G protein-coupled receptor family. Members of this superfamily are characterized by a signature 7-transmembrane domain motif. The specific function of this protein is unknown; however, this protein may mediate the cellular effects of retinoic acid on the G protein signal transduction cascade.

Product Categories/Family for anti-GPRC5B antibody

Antibodies; G Protein Receptors

References

Yokoyama, K., et al. Nephron Clin Pract 115 (4), C237-C243 (2010): Robbins, M.J., et al. Genomics 67(1):8-18(2000). Brauner-Osborne, H., et al. Genomics 65(2):121-128(2000). Loftus, B.J., et al. Genomics 60(3):295-308(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 7706451
• NCBI GeneID: 51704
• NCBI Accession #: NP_057319.1
• NCBI GenBank Nucleotide #: NM_016235.1
• UniProt Accession #: Q9NZH0; O75205; Q8NBZ8
• Molecular Weight: 44,795 Da
• NCBI Official Full Name: G-protein coupled receptor family C group 5 member B
• NCBI Official Synonym Full Names: G protein-coupled receptor, family C, group 5, member B
• NCBI Official Symbol: GPRC5B
• NCBI Official Synonym Symbols: RAIG2; RAIG-2
• NCBI Protein Information: G-protein coupled receptor family C group 5 member B; A-69G12.1; retinoic acid-induced gene 2 protein; retinoic acid responsive gene protein; G protein-coupled receptor, family C, group 1, member B
• UniProt Protein Name: G-protein coupled receptor family C group 5 member B
• Protein Family: G-protein coupled receptor family
• UniProt Gene Name: GPRC5B
• UniProt Synonym Gene Names: RAIG2; RAIG-2
• UniProt Entry Name: GPC5B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the type 3 G protein-coupled receptor family. Members of this superfamily are characterized by a signature 7-transmembrane domain motif. The specific function of this protein is unknown; however, this protein may mediate the cellular effects of retinoic acid on the G protein signal transduction cascade. [provided by RefSeq, Jul 2008]

Uniprot Description

GPRC5B: Unknown. This retinoic acid-inducible G-protein coupled receptor provide evidence for a possible interaction between retinoid and G-protein signaling pathways. Belongs to the G-protein coupled receptor 3 family.

Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 3

Chromosomal Location of Human Ortholog: 16p12

Cellular Component: extracellular space; cell surface; cytoplasmic vesicle membrane; intracellular membrane-bound organelle; nucleolus; plasma membrane; integral to membrane; nucleus; lipid raft

Molecular Function: G-protein coupled receptor activity; G-protein-coupled receptor binding; protein kinase activator activity; protein kinase binding

Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; locomotory behavior; positive regulation of neuron differentiation; glucose homeostasis; positive regulation of inflammatory response