Interleukin-11 ELISA Kit | IL-11 elisa kit

Interleukin-11 (IL-11)

Cat. Num. AAA590016

• Gene Names: IL11; AGIF; IL-11
• Synonyms: Interleukin-11; Interleukin-11 (IL-11); IL-11 elisa kit

• Specificity: This sandwich ELISA can detect both natural and recombinant human IL-11. The following factors were assayed for cross-reactivity at 50 ng/mL in Calibrator Diluent I and Calibrator Diluent II. Preparations of the following factors at 50 ng/mL in a mid-range rhIL-11Control were tested for interference. No significant cross-reactivity or interference was identified.
• Sequence Length: 199

• Samples: Serum, plasma, and Cell Culture Supernatant
• Assay Type: Sandwich
• Sensitivity: The minimum detectable quantities of human IL-10 as observed by the standard curve generated for both Calibrator Diluent I and Calibrator Diluent II are 4.0 pg/mL and 3.0pg/mL respectively. The two standard deviations above the mean optical density of the 16 replicates of the zero standard were defined as the minimum detectable quantities.
• Intra-assay Precision: To determine within-run precision, three different samples of known concentration were assayed by using 16 replicates in 1 assay.
• Inter-assay Precision: To determine between-run precision, three different samples of known concentration were assayed by using replicates on 16 different assays.
• Intended Uses: This Human IL-11 ELISA kit is to be used for the in vitro quantitative determination of human Interleukin 11 (IL-11) concentrations in serum, plasma, and cell culture supernatant. This kit is intended for LABORATORY RESEARCH USE ONLY and is not for use in therapeutic procedures.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-11 elisa kit

Principle of the assay: This IL-11 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-11. Standards or samples are then added to the appropriate microtiter plate wells and incubated. After washing to remove unbound IL-11 and other components of the sample, biotin-conjugated polyclonal antibody specific to IL-11 is added and incubated. If present, IL-11 will bind and become immobilized by the antibody pre-coated on the wells and then become "sandwiched" by the biotin conjugate. In order to quantitatively determine the amount of IL-11 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits, each having a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-11, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in colour. The enzymesubstrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. In order to measure the concentration of IL-11 in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing). According to the testing system, the standard provided is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-11 concentration (pg/mL). The concentration of IL-11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for IL-11 elisa kit

ELISA Kits for Human Cytokines and Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 10834994
• NCBI GeneID: 3589
• NCBI Accession #: NP_000632.1
• NCBI GenBank Nucleotide #: NM_000641.3
• Molecular Weight: 12,994 Da
• NCBI Official Full Name: interleukin-11 isoform 1
• NCBI Official Synonym Full Names: interleukin 11
• NCBI Official Symbol: IL11
• NCBI Official Synonym Symbols: AGIF; IL-11
• NCBI Protein Information: interleukin-11; adipogenesis inhibitory factor; oprelvekin
• UniProt Protein Name: Interleukin-11
• Protein Family: Interleukin
• UniProt Gene Name: IL11
• UniProt Entry Name: IL11_HUMAN

NCBI Description

The protein encoded by this gene is a member of the gp130 family of cytokines. These cytokines drive the assembly of multisubunit receptor complexes, all of which contain at least one molecule of the transmembrane signaling receptor IL6ST (gp130). This cytokine is shown to stimulate the T-cell-dependent development of immunoglobulin-producing B cells. It is also found to support the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2012]

Uniprot Description

IL11: Directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Belongs to the IL-6 superfamily.

Protein type: Cell cycle regulation; Cytokine; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: growth factor activity; cytokine activity; interleukin-11 receptor binding

Biological Process: fat cell differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of MAPKKK cascade; cell-cell signaling; megakaryocyte differentiation; negative regulation of hormone secretion; B cell differentiation; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation