HMGB1 Monoclonal Antibody | anti-HMGB1 antibody

HMGB1, Rat, mAb 5H6

Cat. Num. AAA584778

• Gene Names: HMGB1; HMG1; HMG3; HMG-1; SBP-1
• Reactivity: Rat, Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Synonyms: HMGB1; Monoclonal Antibody; Rat; mAb 5H6; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; SBP-1; anti-HMGB1 antibody

• Host: Armenian Hamster
• Reactivity: Rat, Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 5H6
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 215

• Applicable Applications for anti-HMGB1 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Flow Cytometry: For staining cells were permeabilized with buffer containing 0.5% saponin. The cells were fixed in 0.01% formaldehyde before staining. Hamster IgG isotype control is used as a negative control.; The typical starting working dilution is 1:50.
• Preparation and Storage: Store at 4 degree C. Under recommended storage conditions, product is stable for at least one year.

Related Product Information for anti-HMGB1 antibody

HMGB1 (High Mobility Group Box-1 protein also known as amphoterin) is a highly conserved protein with more than 95% amino acid identity between rodent and human HMGB1. It is a prevalent non-histone chromatin component and a non-sequence specific DNA binding protein. HMGB1 consists of two homologous HMG boxes rich in basic amino acids and an acidic tail at the carboxy-terminus. HMGB1 is involved in the regulation of chromatin structure as well as being involved, either as positive or negative factors with various aspects of DNA replication, transcription, repair, and ligation. HMGB1, identified as a membrane associated protein termed œamphoterin,  mediates neurite outgrowth, tumor outgrowth, and metastasis. It participates in plasminogen activation and is recognized as a late mediator of endotoxin lethality in mice. The monoclonal antibody anti HMGB1, clone 5H6, can be used for Western blot, Flow cytometry and Immunofluorescence.

NCBI and Uniprot Product Information

• NCBI GI #: 926459557
• NCBI GeneID: 3146
• NCBI Accession #: NP_001300822.1
• NCBI GenBank Nucleotide #: NM_001313893.1
• UniProt Accession #: P09429; Q14321; Q5T7C3; Q6IBE1; A5D8W9
• Molecular Weight: 24,894 Da
• NCBI Official Full Name: high mobility group protein B1
• NCBI Official Synonym Full Names: high mobility group box 1
• NCBI Official Symbol: HMGB1
• NCBI Official Synonym Symbols: HMG1; HMG3; HMG-1; SBP-1
• NCBI Protein Information: high mobility group protein B1
• UniProt Protein Name: High mobility group protein B1
• Protein Family: High mobility group protein
• UniProt Gene Name: HMGB1
• UniProt Synonym Gene Names: HMG1; HMG-1

NCBI Description

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]

Uniprot Description

HMGB1: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Belongs to the HMGB family.

Protein type: DNA repair, damage; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 13q12.3

Cellular Component: cell surface; condensed chromosome; extracellular region; extracellular space; nucleoplasm; nucleus; transcriptional repressor complex

Molecular Function: bubble DNA binding; C-X-C chemokine binding; chemoattractant activity; cytokine activity; damaged DNA binding; DNA bending activity; double-stranded DNA binding; four-way junction DNA binding; lipopolysaccharide binding; lyase activity; phosphatidylserine binding; protein binding; RAGE receptor binding; single-stranded DNA binding; transcription factor activity; transcription factor binding

Biological Process: activation of innate immune response; apoptotic cell clearance; dendritic cell chemotaxis; DNA fragmentation during apoptosis; DNA geometric change; DNA ligation during DNA repair; DNA recombination; DNA topological change; elevation of cytosolic calcium ion concentration; inflammatory response; inflammatory response to antigenic stimulus; innate immune response; myeloid dendritic cell activation; negative regulation of blood vessel endothelial cell migration; negative regulation of CD4-positive, alpha beta T cell differentiation; negative regulation of interferon-gamma production; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcriptional preinitiation complex assembly; neurite development; positive regulation of activated T cell proliferation; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of DNA binding; positive regulation of DNA ligation; positive regulation of interleukin-1 secretion; positive regulation of interleukin-10 production; positive regulation of interleukin-12 production; positive regulation of JNK cascade; positive regulation of MAPKKK cascade; positive regulation of mismatch repair; positive regulation of toll-like receptor 9 signaling pathway; positive regulation of transcription from RNA polymerase II promoter; regulation of autophagy; regulation of restriction endodeoxyribonuclease activity; regulation of T cell mediated immune response to tumor cell; regulation of tolerance induction; regulation of transcription from RNA polymerase II promoter; T-helper 1 cell differentiation; V(D)J recombination