Mouse anti-Human CD83 Monoclonal Antibody | anti-CD83 antibody

CD83, Human, mAb ELBE-1

Cat. Num. AAA584768

• Gene Names: CD83; BL11; HB15
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Synonyms: CD83; Monoclonal Antibody; Human; mAb ELBE-1; B-cell activation protein; Cell surface protein HB15; anti-CD83 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: ELBE-1
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 205

• Applicable Applications for anti-CD83 antibody: Flow Cytometry (FC/FACS), Immuno Assay (IA), Western Blot (WB)
• Application Notes: Flow Cytometry: Antibody ELBE-1 stains the extracellular domain of CD83.
• Immunogen: huCD83Ig fusion protein
• Positive Control: PMBCs Double staining with CD11c-PE
• Preparation and Storage: Store at 4 degree C.

Related Product Information for anti-CD83 antibody

Monoclonal antibody ELBE-1 recognizes human CD83. CD83 is a cell surface marker predominantly expressed on mature DCs. Expression has been found on circulating and tissue DCs. Additionally CD83 is found on activated B and T lymphocytes. Human CD83 is composed of 205 amino acids but has, due to extensive glycosylation, a molecular weight of ca 45 kDa. CD83 is expressed as a conserved a noncovalently associated single chain molecule membrane protein. CD83 is considered a marker for fully matured dendritic cells in the peripheral circulation. An essential role has not been found. However, its function primarily lies in the regulation of T- and B-cell maturation and the regulation of their peripheral responses. CD83 is supposed to function as a co-stimulator for T-cell activation similar to CD80 or CD86. Enhanced expression on DCs correlates with their T cell activating capacity. Besides the membrane version of the protein also a soluble form occurs (sCD83). sCD83 levels are raised in sera from patients of chronic lymphocytic leukemia and other haematological malignant diseases and in the synovial fluid of rheumatoid arthritis. While the membrane bound form has immune stimulatory capacity, the soluble form has the opposite effect and is considered to have immunosuppressive potential. Due to its inhibitory functions, sCD83 has potential for treatment of eg. autoimmune diseases and transplantation. In contradiction to most clones, antibody ELBE-1 is besides flow cytometry applicable in western blotting and ELISA (detecting of sCD83). ELBE-1 recognizes a linear epitope and doesn't influence T cell activation.

References

1. Papp, S et al. A New Monoclonal Anti-human CD83 Antibody for Flow Cytometry, Western Blot Analysis, and ELISA. Monoclon Antib Immunodiagn Immunother 2013, 32: 98.

NCBI and Uniprot Product Information

• NCBI GI #: 4757946
• NCBI GeneID: 9308
• NCBI Accession #: NP_004224.1
• NCBI GenBank Nucleotide #: NM_004233.3
• UniProt Accession #: Q01151; Q5THX9
• Molecular Weight: 23,042 Da
• NCBI Official Full Name: CD83 antigen isoform a
• NCBI Official Synonym Full Names: CD83 molecule
• NCBI Official Symbol: CD83
• NCBI Official Synonym Symbols: BL11; HB15
• NCBI Protein Information: CD83 antigen
• UniProt Protein Name: CD83 antigen
• Protein Family: CD83 antigen
• UniProt Gene Name: CD83
• UniProt Synonym Gene Names: hCD83

NCBI Description

The protein encoded by this gene is a single-pass type I membrane protein and member of the immunoglobulin superfamily of receptors. The encoded protein may be involved in the regulation of antigen presentation. A soluble form of this protein can bind to dendritic cells and inhibit their maturation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

CD83: May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p23

Cellular Component: integral to plasma membrane; plasma membrane

Biological Process: defense response; humoral immune response; regulation of cytokine production; signal transduction