Human Lactoferrin ELISA Kit | LTF elisa kit

Lactoferrin, Human, ELISA Kit

Cat. Num. AAA584537

• Gene Names: LTF; LF; HLF2; GIG12; HEL110
• Reactivity: Human
• Synonyms: Lactoferrin; Human; ELISA Kit; LTF elisa kit

• Reactivity: Human
• Sequence Length: 475

• Application Notes: Application Notes: The Human Lactoferrin ELISA Kit has been developed for the quantitative measurement of natural and recombinant human lactoferrin in cell culture medium, plasma, breast milk, faeces, urine and other body fluids.
• Detection Level: 0.4 ng/ml
• Standard Range: 0.4-100 ng/ml
• Working Volume: 100 ul/well
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for LTF elisa kit

Product Description: Human lactoferrin (LF) is an 80 kDa glycoprotein found concentrated in the secondary granules of the neutrophils. In addition, lactoferrin can be found in epithelia and most body fluids and secretions. Lactoferrin was first isolated from human milk and plays an important part in the immune system by helping to fight infections. It has the ability to bind iron and possesses five different enzyme activities: DNase, RNase, ATPase, phosphatase, and malto-oligosaccharide hydrolysis. Lactoferrin is a natural anti-bacterial, anti-fungal and anti-viral protein, it is an antioxidant and also possesses immunomodulatory properties. Furthermore, lactoferrin promotes the health of the gastro-intestinal system by improving the intestinal microbalance. Lactoferrin is secreted in plasma by neutrophils. Plasma of healthy individuals contains ~190-500 ng/ml LF. The lactoferrin plasma concentration represents a positive relation to the total pool of neutrophils and the rate of neutrophil turnover. Upon inflammation, lactoferrin is released from the secondary neutrophil granules into the extracellular medium. Therefore, the extracellular lactoferrin concentration can be used as an index for neutrophil activation. The iron binding property of lactoferrin is considered to be an important antimicrobial function. Human lactoferrin binds via its highly positively charged amino-terminus to bacterial products. It kills various bacteria, most probably by inducing intracellular changes in these bacteria without affecting the membrane permeability. Cleavage by pepsin of lactoferrin leads to the release of lactoferricin H. This 47 amino acid peptide has more antimicrobial activity than its precursor and it can inhibit the classical but not the alternative complement pathway. Urine or breast milk of healthy persons contain ~30 ng/ml and ~500 ug/ml LF, respectively. During infection, the LF concentration can raise 10-100-fold. In faeces of healthy persons, ~1 ug/g LF can be detected, whereas in faeces derived from colon cancer or inflammatory bowel disease (IBD) patients, LF levels range from ~75-310 ug/g. Faecal lactoferrin is useful as a sensitive and specific marker in identifying intestinal inflammation such as Crohn's disease and IBD. Combination of several markers, such as calprotectin, defensin, elastase, MPO, I-FABP and MAdCAM, may be useful for classifying IBD, as well as for identifying tumor grade and to confirm remission/response to treatment. Therefore, the Human Lactoferrin ELISA is a sensitive, non-invasive tool for monitoring disease activity.
Principle of the assay: The human lactoferrin ELISA Kit is a solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours.

NCBI and Uniprot Product Information

• NCBI GI #: 2104522
• NCBI GeneID: 4057
• Molecular Weight: 73,161 Da
• NCBI Official Full Name: lactoferrin, partial
• NCBI Official Synonym Full Names: lactotransferrin
• NCBI Official Symbol: LTF
• NCBI Official Synonym Symbols: LF; HLF2; GIG12; HEL110
• NCBI Protein Information: lactotransferrin; talalactoferrin; neutrophil lactoferrin; growth-inhibiting protein 12; epididymis luminal protein 110
• UniProt Protein Name: Lactotransferrin
• Protein Family: Lactotransferrin
• UniProt Gene Name: LTF
• UniProt Synonym Gene Names: GIG12; LF; Lactoferrin; Lfcin-H
• UniProt Entry Name: TRFL_HUMAN