Rat anti-Mouse Macrophages F4/80 Monoclonal Antibody
Macrophages F4/80, Mouse, mAb BM8
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Western Blot
Synonyms
Macrophages F4/80; Monoclonal Antibody; Mouse; mAb BM8; anti-Macrophages F4/80 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2a
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Applicable Applications for anti-Macrophages F4/80 antibody
F, Flow Cytometry (FC/FACS), P, Western Blot (WB
Application Notes
Application Notes: W: Mouse bone-marrow derived macrophages; non-reduced; ~125 kDa (Ref 1); reduction with 2-mercaptoethanol destroys BM8 antigen
Application Use: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. F4/80 is sensitive to 2-mercaptoethanol. The antibody requires proteinase K treatment of paraffin sections.
Application Use: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. F4/80 is sensitive to 2-mercaptoethanol. The antibody requires proteinase K treatment of paraffin sections.
Immunogen
BALB/c macrophages obtained from 14-day-old bone marrow cell cultures
Positive Control
Mouse macrophages
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-Macrophages F4/80 antibody
Product Description: The monoclonal antibody BM8 recognises a 125 kD extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. This murine F4/80 glycoprotein contains seven-transmembrane (TM7) regions, which anchor the protein in the cell membrane, and thereby shows similarity in this region to G-protein-coupled receptors. The F4/80 molecule shares overall structural homology to other members of the epidermal growth factor (EGF)-TM7 family. The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. It is absent on granulocytes, lymphocytes and trombocytes. The expression of F4/80 increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny. The monoclonal antibody BM8 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model. The monoclonal antibody BM8 cross reacts with human heart macrophages.
References
1. Malorny, U et al; A monoclonal antibody against an antigen present on mouse macrophages and absent from monocytes. Cell Tissue Res 1986, 243: 421 2. Leenen, P et al; Markers of mouse macrophage development detected by monoclonal antibodies. J Immunol Methods 1994, 174: 5
3. Mackler, A et al; Macrophage trafficking in the uterus and cervix precedes parturition in the mouse. Biol Reprod 1999, 61: 879
4. Schaller, E et al; Inactivation of the F4/80 glycoprotein in the mouse germ line. Mol Cell Biol 2002, 22: 8035
3. Mackler, A et al; Macrophage trafficking in the uterus and cervix precedes parturition in the mouse. Biol Reprod 1999, 61: 879
4. Schaller, E et al; Inactivation of the F4/80 glycoprotein in the mouse germ line. Mol Cell Biol 2002, 22: 8035
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Product Notes
The Macrophages F4/80 (Catalog #AAA584306) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Macrophages F4/80, Mouse, mAb BM8 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Macrophages F4/80 can be used in a range of immunoassay formats including, but not limited to, F, Flow Cytometry (FC/FACS), P, Western Blot (WB. Application Notes: W: Mouse bone-marrow derived macrophages; non-reduced; ~125 kDa (Ref 1); reduction with 2-mercaptoethanol destroys BM8 antigen Application Use: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. F4/80 is sensitive to 2-mercaptoethanol. The antibody requires proteinase K treatment of paraffin sections. Researchers should empirically determine the suitability of the Macrophages F4/80 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Macrophages F4/80, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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