Mouse anti-Human CD59 Monoclonal Antibody | anti-CD59 antibody
CD59, Human, mAb MEM-43
Reactivity
Human
Applications
Western Blot
Synonyms
CD59; Monoclonal Antibody; Human; mAb MEM-43; anti-CD59 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2a
Form/Format
Sterile 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS and 0.1% bovine serum albumin with low endotoxin level.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Applicable Applications for anti-CD59 antibody
Western Blot (WB)
Application Notes
Application Use: For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity dilutions have to be made according to the amounts endotoxin to be inactivated.
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-CD59 antibody
Product Description: The antibody is the prototypic antibody for the CD59 determinant, a PI-linked glycoprotein of 18-25 kDa, which is broadly expressed on most types of cells. The antigen is the major complement-protecting molecule of human cells. It is expressed on all leukocytes, erythrocytes and most other cell types. Binding of this antibody inhibits the complement-protecting activity of CD59. CD59 is one of the complement regulatory membrane proteins that is missing in Paroxysmal Nocturnal Haemoglobinuria (PNH). Anti CD59 reagents may be useful in the differential identification of PNH type II and PNH type I. Recently a role for CD59 in LPS signal transduction has been reported in keratinocytes. The antibody reacts with well defined epitope (W40, R-53) on CD59 molecule.
References
1. Yakamoto, T et al; Lipopolysaccharide signal transduction in oral keratinocytes - involvement of CD59 but not CD14. Cellular Signalling 2003, 15: 861 2. Horesji, V et al; Folia Biol 1986, 34: 12
3. Knapp, W et al; Leucocyte Typing IV, Oxford University Press 1989
4. Schlossman, S et al; Leucocyte Typing IV, Oxford University Press 1995
5. Cebecauer, M et al; Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells. Biochem Biophys Res Commun 1998, 234: 1998
3. Knapp, W et al; Leucocyte Typing IV, Oxford University Press 1989
4. Schlossman, S et al; Leucocyte Typing IV, Oxford University Press 1995
5. Cebecauer, M et al; Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells. Biochem Biophys Res Commun 1998, 234: 1998
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Product Notes
The CD59 (Catalog #AAA584198) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD59, Human, mAb MEM-43 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD59 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Application Use: For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity dilutions have to be made according to the amounts endotoxin to be inactivated. Researchers should empirically determine the suitability of the CD59 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD59, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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