Rabbit anti-Human ANKS1B Polyclonal Antibody | anti-ANKS1B antibody

ANKS1B, ID (ANKS1B, Ankyrin repeat and sterile alpha motif domain-containing protein 1B, Amyloid-beta protein intracellular domain-associated protein 1, E2A-PBX1-associated protein) (AP)

Cat. Num. AAA6273752

• Gene Names: ANKS1B; EB1; AIDA; EB-1; ANKS2; AIDA-1; cajalin-2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ANKS1B; Polyclonal Antibody; ID (ANKS1B; Ankyrin repeat and sterile alpha motif domain-containing protein 1B; Amyloid-beta protein intracellular domain-associated protein 1; E2A-PBX1-associated protein) (AP); E2A-PBX1-associated protein; anti-ANKS1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-ANKS1B antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ANKS1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 569-599 amino acids from the Central region of human ANKS1B.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ANKS1B Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the ANKS1B antibody detected the ANKS1B protein (arrow).)

Immunohistochemistry (IHC)

(ANKS1B Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ANKS1B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-ANKS1B antibody

Isoform 2 may participate in the regulation of nucleoplasmic coilin protein interactions in neuronal and transformed cells. Isoform 3 can regulate global protein synthesis by altering nucleolar numbers (By similarity). Isoform 4 may play a role as a modulator of APP processing. Overexpression can down-regulate APP processing.

Product Categories/Family for anti-ANKS1B antibody

Antibodies; Ankyrin

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Pei, D., et al. Genet Test Mol Biomarkers 14(3):433-438(2010). Wang, M., et al. Stat Biopharm Res 1(4):424-430(2009). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 10047134
• NCBI GeneID: 56899
• NCBI Accession #: NP_064525.1; NP_690001.3
• NCBI GenBank Nucleotide #: NM_020140.3
• UniProt Accession #: Q7Z6G8; Q5XLJ0; Q6IVB5; A5PKY5; A7E259; A8K153; A8MSN4; B4DFP6; B4DH98; F8VPM3; F8VZR9; F8WC27
• Molecular Weight: 44,522 Da
• NCBI Official Full Name: ankyrin repeat and sterile alpha motif domain-containing protein 1B isoform c
• NCBI Official Synonym Full Names: ankyrin repeat and sterile alpha motif domain containing 1B
• NCBI Official Symbol: ANKS1B
• NCBI Official Synonym Symbols: EB1; AIDA; EB-1; ANKS2; AIDA-1; cajalin-2
• NCBI Protein Information: ankyrin repeat and sterile alpha motif domain-containing protein 1B; cajalin 2; E2a-Pbx1-associated protein; amyloid-beta precursor protein intracellular domain associated protein 1
• UniProt Protein Name: Ankyrin repeat and sterile alpha motif domain-containing protein 1B
• Protein Family: Ankyrin repeat and sterile alpha motif domain-containing protein
• UniProt Gene Name: ANKS1B
• UniProt Synonym Gene Names: AIDA-1; EB-1
• UniProt Entry Name: ANS1B_HUMAN

NCBI Description

This gene encodes a multi-domain protein that is predominantly expressed in brain and testis. This protein interacts with amyloid beta protein precursor (AbetaPP) and may have a role in normal brain development, and in the pathogenesis of Alzheimer's disease. Expression of this gene has been shown to be elevated in patients with pre-B cell acute lymphocytic leukemia associated with t(1;19) translocation. Alternatively spliced transcript variants encoding different isoforms (some with different subcellular localization, PMID:15004329) have been described for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

AIDA-1b: Isoform 2 may participate in the regulation of nucleoplasmic coilin protein interactions in neuronal and transformed cells. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion

Chromosomal Location of Human Ortholog: 12q23.1

Cellular Component: Cajal body; postsynaptic membrane; cytoplasm; postsynaptic density; dendritic spine; cell junction

Molecular Function: ephrin receptor binding