Rat anti-Mouse Endomucin Monoclonal Antibody | anti-EMCN antibody

Endomucin, Mouse, mAb V.7C7.1

Cat. Num. AAA584182

• Gene Names: EMCN; EMCN2; MUC14
• Reactivity: Mouse
• Applications: Western Blot
• Synonyms: Endomucin; Monoclonal Antibody; Mouse; mAb V.7C7.1; Endomucin-2; mucin-14; gastric cancer antigen Ga34; anti-EMCN antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2a
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 261

• Applicable Applications for anti-EMCN antibody: P, Western Blot (WB)
• Application Notes: Application Use: For flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
• Immunogen: Mouse endomucin-IgG fusion protein
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-EMCN antibody

Product Description: The monoclonal antibody V.7C7.1 recognizes endomucin, type I membrane protein of 248 amino acids (75 kDa) and shows no signicant homology to any known glycoprotein. As a typical mucin-like glycoprotein, endomucin has a high content of serine and threonine residues, suggesting strong O-glycosylation; the sensitivity to O-sialoglycoprotein endopeptidase indicates that endomucin is also a sialomucin. Endomucin is an endothelial-specific sialomucin. It is a constitutively expressed endothelial cell surface protein that is found on all venules but is absent from high endothelial venule cells (HEV) of peripheral and mesenteric lymph nodes as well as Peyer's patches, the specialized site for most efficient lymphocyte trafficking. This could indicate an anti-adhesive function of endomucin, as demonstrated for other sialomucins. Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) is another cell adhesion molecule that contains a mucin-like domain and is expressed on HEV in Peyer's patches, mesenteric lymph nodes and on venules in intestinal lamina propria. In the HEV of mesenteric lymph nodes, the mucin-like domain of a subpopulation of MAdCAM-1 molecules contains sulfated carbohydrate side chains that interact with L-selectin. The presence of three putative protein kinase C phosphorylation sites in the cytoplasmic tail of endomucin indicates that endomucin has the capacity to be a signaling molecule.

References

1. Morgan, S et al; Biochemical characterization and molecular cloning of a novel endothelial-specific sialomucin. Blood 1999, 93: 165 2. Kuhn, A et al; Expression of endomucin, a novel endothelial sialomucin, in normal and diseased skin. J Invest Dermatol. 2002, 119: 1388

NCBI and Uniprot Product Information

• NCBI GI #: 20070284
• NCBI GeneID: 51705
• NCBI Accession #: NP_057326.2
• NCBI GenBank Nucleotide #: NM_016242.3
• Molecular Weight: 26,066 Da
• NCBI Official Full Name: endomucin isoform 1
• NCBI Official Synonym Full Names: endomucin
• NCBI Official Symbol: EMCN
• NCBI Official Synonym Symbols: EMCN2; MUC14
• NCBI Protein Information: endomucin; MUC-14; mucin-14; endomucin-2; gastric cancer antigen Ga34
• UniProt Protein Name: Endomucin
• Protein Family: Endomucin
• UniProt Gene Name: EMCN
• UniProt Synonym Gene Names: EMCN2; MUC14; MUC-14
• UniProt Entry Name: MUCEN_HUMAN

NCBI Description

EMCN is a mucin-like sialoglycoprotein that interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix (Kinoshita et al., 2001 [PubMed 11418125]).[supplied by OMIM, Mar 2008]

Uniprot Description

EMCN: Endothelial sialomucin, also called endomucin or mucin- like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: plasma membrane; integral to membrane; extracellular region