Western Blot (WB)
(Western Blot detection against Immunogen (36.74kD).)
Mouse anti-Human, Rat CITED1 Monoclonal Antibody | anti-CITED1 antibody
CITED1 (CBP/p300 Interacting Transactivator 1, Cbp/p300-interacting Transactivator with Glu/Asp-rich Carboxy-terminal Domain 1, Melanocyte-specific Protein1)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Western Blot (WB)
(Western Blot detection against Immunogen (36.74kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (36.74kD).)
Western Blot (WB)
(CITED1 monoclonal antibody. Western Blot analysis of CITED1 expression in PC-12.)
Western Blot (WB)
(CITED1 monoclonal antibody. Western Blot analysis of CITED1 expression in PC-12.)
Western Blot (WB)
(CITED1 monoclonal antibody Western Blot analysis of CITED1 expression in A-431.)
Western Blot (WB)
(CITED1 monoclonal antibody Western Blot analysis of CITED1 expression in A-431.)
Western Blot (WB)
(Western Blot analysis of CITED1 expression in transfected 293T cell line by CITED1 monoclonal antibody.|Lane 1: CITED1 transfected lysate (19.9kD).|Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of CITED1 expression in transfected 293T cell line by CITED1 monoclonal antibody.|Lane 1: CITED1 transfected lysate (19.9kD).|Lane 2: Non-transfected lysate.)
Testing Data
(Detection limit for recombinant GST tagged CITED1 is ~0.3ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged CITED1 is ~0.3ng/ml as a capture antibody.)
Western Blot (WB)
(Western blot analysis of CITED1 over-expressed 293 cell line, cotransfected with CITED1 Validated Chimera RNAi ((Lane 2) or non-transfected control (Lane 1). Blot probed with CITED1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Western Blot (WB)
(Western blot analysis of CITED1 over-expressed 293 cell line, cotransfected with CITED1 Validated Chimera RNAi ((Lane 2) or non-transfected control (Lane 1). Blot probed with CITED1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the CREB-binding protein/p300-interacting transactivator with Asp/Glu-rich C-terminal domain (CITED) family of proteins. The encoded protein, also known as melanocyte-specific gene 1, may function as a transcriptional coactivator and may play a role in pigmentation of melanocytes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]
Uniprot Description
CITED1: Transcriptional coactivator of the p300/CBP-mediated trancription complex. Enhances SMAD-mediated transcription by strengthening the functional link between the DNA-binding SMAD transcription factors and the p300/CBP transcription coactivator complex. Stimulates estrogen-dependent transactivation activity mediated by estrogen receptors signaling; stabilizes the interaction of estrogen receptor ESR1 and histone acetyltransferase EP300. Positively regulates TGF-beta signaling through its association with the SMAD/p300/CBP-mediated transcriptional coactivator complex. Induces transcription from estrogen-responsive promoters and protection against cell death. Potentiates EGR2-mediated transcriptional activation activity from the ERBB2 promoter. Acts as an inhibitor of osteoblastic mineralization through a cAMP-dependent parathyroid hormone receptor signaling. May play a role in pigmentation of melanocytes. Associates with chromatin to the estrogen-responsive TGF-alpha promoter region in a estrogen-dependent manner. Belongs to the CITED family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: Xq13.1
Cellular Component: cytoplasm; nucleus; cytosol
Molecular Function: protein C-terminus binding; protein binding; protein homodimerization activity; transcription coactivator activity; LBD domain binding; chromatin binding; transcription factor activity
Biological Process: negative regulation of Wnt receptor signaling pathway; response to cAMP; apoptosis; positive regulation of transcription, DNA-dependent; nucleocytoplasmic transport; response to lipopolysaccharide; response to insulin stimulus; transforming growth factor beta receptor signaling pathway; melanocyte differentiation; negative regulation of osteoblast differentiation; negative regulation of neuron apoptosis; vasculogenesis; placenta development; transcription, DNA-dependent; embryonic axis specification; positive regulation of transforming growth factor beta receptor signaling pathway; melanin biosynthetic process; regulation of transcription from RNA polymerase II promoter; cell proliferation; pigmentation; ureteric bud branching; response to estrogen stimulus; response to cytokine stimulus; brain development; negative regulation of transcription, DNA-dependent; metanephros development
Research Articles on CITED1
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Product Notes
The CITED1 cited1 (Catalog #AAA6008420) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CITED1 (CBP/p300 Interacting Transactivator 1, Cbp/p300-interacting Transactivator with Glu/Asp-rich Carboxy-terminal Domain 1, Melanocyte-specific Protein1) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CITED1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Researchers should empirically determine the suitability of the CITED1 cited1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SMQLQKLNSQ YQGMAAATPG QPGEAGPLQN WDFGAQAGGA ESLSPSAGAQ SPAIIDSDPV DEEVLMSLVV ELGLDRANEL PELWLGQNEF DFTADFPSSC. It is sometimes possible for the material contained within the vial of "CITED1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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