Rat anti-Mouse C5aR Monoclonal Antibody | anti-C5aR antibody

C5aR, Mouse, mAb 20/70

Cat. Num. AAA584113

• Reactivity: Mouse
• Applications: Flow Cytometry, Immunofluorescence
• Synonyms: C5aR; Monoclonal Antibody; Mouse; mAb 20/70; Complement component 5a receptor; CD88; C5r1; C5aR1; rhodopsin-like receptor; C5a anaphylatoxin chemotactic receptor; anti-C5aR antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2b
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)

• Applicable Applications for anti-C5aR antibody: Flow Cytometry (FC), FS, Immunofluorescence (IF)
• Application Notes: Application Notes: FC: 2x105 cells were blocked with human and murine IgG on ice for 20';. Cells were stained with 10 ug antibody20/70 for 45'; in PBS/1.5%FCS. For analysis with FACS cells were washed with PBS/1%formaldehyde (Ref.2).; Application Use: For flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of mouse C5aR to be inactivated.
• Immunogen: RBL-2H3 transfectantsexpressing murine C5aR on the cell surface.
• Positive Control: Mouse neutrophils
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-C5aR antibody

Product Description: The monoclonal antibody 20/70 recognizes the mouse receptor for C5a (CD88). The mouse anaphylatoxin complement factor C5a, a split product of C5, is one of the most potent inflammatory chemoattractants and interacts with two C51 receptors, C5aR and C5L2.- Most of the C5a effects are mediated through C5aR. C5aR is known to be crucial in the initiation of acute inflammatory responses. C5a receptor is a seven transmembrane GTP-binding-protein-coupled receptor that belongs to the rhodopsin supergene family. The 45 kDa C5a receptor is expressed by leukocytes such as neutrophils, eosinophils, basophils, monocytes, dendritic cells and mast cells. C5aR can be expressed on both immune and nonimmune cells. Nonimmune cells expressing C5aR include vascular endothelial cells, cardiomyocytes and bronchial epithelial cells. Signaling of C5aR involves intracellular calcium mobilization and activation of among others protein kinase- B (PKB) and protein phospholipase D (PLD) pathways. High C5aR expression levels have been associated with the pathogenesis of many inflammatory conditions, autoimmune and neurodegenerative diseases like rheumatoid arthritis, multiple sclerosis, atherosclerosis and inflammatory bowel diseases. In vivo blockade of C5aR greatly reduces inflammatory injury. The monoclonal antibody 20/70 has been used in functional studies and is capable- to prevent the binding of C5a to its receptor.

References

1. Shushakova, N et al; C5a anaphylatoxin is a major regulator of activating versus inhibitory FcγRs in immune complex-induced lung disease. J Clin Invest 2002, 110: 1823 2. Soruri, A et al; Characterization of C5aR expression on murine myeloid and lymphoid cells by the use of a novel monoclonal antibody. Immunol Lett 2003, 88: 47
3. Godau, J et al; C5a initiates the inflammatory cascade in immune complex peritonitis. J Immunol 2004, 173: 3437
4. Chen, NJ et al; C5L2 is critical for the biological activities of the anaphylatoxins C5a and C3a. Nature 2007, 446: 203
5. Rittirsch, D et al; Functional roles for C5a receptors in sepsis. Nat Med 2008, 14: 551
6. Gueler, F et al; Complement 5a Receptor inhibition improves renal allograft survival. J Am Soc Nephrol 2008, 19: 2302